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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nematostella vectensis Protein ST7 homolog (v1g243132), partial CSB-YP415234NGO1
CSB-EP415234NGO1
CSB-BP415234NGO1
CSB-MP415234NGO1
CSB-EP415234NGO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Anamorsin homolog (v1g167244) CSB-YP415235NGO
CSB-EP415235NGO
CSB-BP415235NGO
CSB-MP415235NGO
CSB-EP415235NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Probable ethanolamine kinase (etnk) CSB-YP415236NGO
CSB-EP415236NGO
CSB-BP415236NGO
CSB-MP415236NGO
CSB-EP415236NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Guanine-nucleotide exchange factor YEL1 (YEL1), partial CSB-YP415237VDW
CSB-EP415237VDW
CSB-BP415237VDW
CSB-MP415237VDW
CSB-EP415237VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Actin cytoskeleton-regulatory complex protein END3 (END3) CSB-YP415238VDW
CSB-EP415238VDW
CSB-BP415238VDW
CSB-MP415238VDW
CSB-EP415238VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Mitochondrial distribution and morphology protein 34 (MDM34), partial CSB-YP415239VDW
CSB-EP415239VDW
CSB-BP415239VDW
CSB-MP415239VDW
CSB-EP415239VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Maintenance of telomere capping protein 6 (MTC6), partial CSB-YP415240VDW
CSB-EP415240VDW
CSB-BP415240VDW
CSB-MP415240VDW
CSB-EP415240VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Protein SOP4 (SOP4), partial CSB-YP415241VDW
CSB-EP415241VDW
CSB-BP415241VDW
CSB-MP415241VDW
CSB-EP415241VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Elongation factor G, mitochondrial (MEF1), partial CSB-YP415242VDW
CSB-EP415242VDW
CSB-BP415242VDW
CSB-MP415242VDW
CSB-EP415242VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Protein LOT5 (LOT5) CSB-YP415243VDW
CSB-EP415243VDW
CSB-BP415243VDW
CSB-MP415243VDW
CSB-EP415243VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Altered inheritance of mitochondria protein 4 (AIM4) CSB-YP415244VDW
CSB-EP415244VDW
CSB-BP415244VDW
CSB-MP415244VDW
CSB-EP415244VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Nucleolar protein 9 (NOP9), partial CSB-YP415245VDW
CSB-EP415245VDW
CSB-BP415245VDW
CSB-MP415245VDW
CSB-EP415245VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Leukotriene A-4 hydrolase homolog (Kpol_1039p41), partial CSB-YP415246VDW
CSB-EP415246VDW
CSB-BP415246VDW
CSB-MP415246VDW
CSB-EP415246VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Spindle pole body component 110 (SPC110), partial CSB-YP415247VDW
CSB-EP415247VDW
CSB-BP415247VDW
CSB-MP415247VDW
CSB-EP415247VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Mitochondrial division protein 1 (MDV1), partial CSB-YP415248VDW
CSB-EP415248VDW
CSB-BP415248VDW
CSB-MP415248VDW
CSB-EP415248VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Solute carrier family 25 member 38 homolog (Kpol_1043p32), partial CSB-YP415249VDW1
CSB-EP415249VDW1
CSB-BP415249VDW1
CSB-MP415249VDW1
CSB-EP415249VDW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora ATP-dependent RNA helicase DBP3 (DBP3), partial CSB-YP415250VDW
CSB-EP415250VDW
CSB-BP415250VDW
CSB-MP415250VDW
CSB-EP415250VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Autophagy-related protein 14 (ATG14) CSB-YP415251VDW
CSB-EP415251VDW
CSB-BP415251VDW
CSB-MP415251VDW
CSB-EP415251VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Regulator of free ubiquitin chains 1 (RFU1) CSB-YP415252VDW
CSB-EP415252VDW
CSB-BP415252VDW
CSB-MP415252VDW
CSB-EP415252VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora ADP-ribose 1'-phosphate phosphatase (POA1) CSB-YP415253VDW
CSB-EP415253VDW
CSB-BP415253VDW
CSB-MP415253VDW
CSB-EP415253VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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