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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Campylobacter jejuni subsp. doylei Protein translocase subunit SecA (secA), partial CSB-YP416894CWE
CSB-EP416894CWE
CSB-BP416894CWE
CSB-MP416894CWE
CSB-EP416894CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei D-alanine--D-alanine ligase (ddl) CSB-YP416895CWE
CSB-EP416895CWE
CSB-BP416895CWE
CSB-MP416895CWE
CSB-EP416895CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 50S ribosomal protein L19 (rplS) CSB-YP416896CWE
CSB-EP416896CWE
CSB-BP416896CWE
CSB-MP416896CWE
CSB-EP416896CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP416897CWE
CSB-EP416897CWE
CSB-BP416897CWE
CSB-MP416897CWE
CSB-EP416897CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Aspartate--tRNA ligase (aspS), partial CSB-YP416898CWE
CSB-EP416898CWE
CSB-BP416898CWE
CSB-MP416898CWE
CSB-EP416898CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Glutamyl-tRNA reductase (hemA) CSB-YP416899CWE
CSB-EP416899CWE
CSB-BP416899CWE
CSB-MP416899CWE
CSB-EP416899CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP416900CWE1
CSB-EP416900CWE1
CSB-BP416900CWE1
CSB-MP416900CWE1
CSB-EP416900CWE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei N utilization substance protein B homolog (nusB) CSB-YP416901CWE
CSB-EP416901CWE
CSB-BP416901CWE
CSB-MP416901CWE
CSB-EP416901CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Tryptophan synthase beta chain (trpB) CSB-YP416902CWE
CSB-EP416902CWE
CSB-BP416902CWE
CSB-MP416902CWE
CSB-EP416902CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Histidinol-phosphate aminotransferase (hisC) CSB-YP416903CWE
CSB-EP416903CWE
CSB-BP416903CWE
CSB-MP416903CWE
CSB-EP416903CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei tRNA pseudouridine synthase D (truD) CSB-YP416904CWE
CSB-EP416904CWE
CSB-BP416904CWE
CSB-MP416904CWE
CSB-EP416904CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP416905CWE
CSB-EP416905CWE
CSB-BP416905CWE
CSB-MP416905CWE
CSB-EP416905CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 30S ribosomal protein S11 (rpsK) CSB-YP416906CWE
CSB-EP416906CWE
CSB-BP416906CWE
CSB-MP416906CWE
CSB-EP416906CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Na (+)/H (+) antiporter nhaA 1, partial CSB-YP416907CWE
CSB-EP416907CWE
CSB-BP416907CWE
CSB-MP416907CWE
CSB-EP416907CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 50S ribosomal protein L18 (rplR) CSB-YP416908CWE
CSB-EP416908CWE
CSB-BP416908CWE
CSB-MP416908CWE
CSB-EP416908CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 50S ribosomal protein L5 (rplE) CSB-YP416909CWE
CSB-EP416909CWE
CSB-BP416909CWE
CSB-MP416909CWE
CSB-EP416909CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 30S ribosomal protein S3 (rpsC) CSB-YP416910CWE
CSB-EP416910CWE
CSB-BP416910CWE
CSB-MP416910CWE
CSB-EP416910CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP416911CWE
CSB-EP416911CWE
CSB-BP416911CWE
CSB-MP416911CWE
CSB-EP416911CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP416912AUT
CSB-EP416912AUT
CSB-BP416912AUT
CSB-MP416912AUT
CSB-EP416912AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP416913AUT
CSB-EP416913AUT
CSB-BP416913AUT
CSB-MP416913AUT
CSB-EP416913AUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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