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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rat C-X-C motif chemokine 2 (Cxcl2) CSB-YP006248RA
CSB-EP006248RA
CSB-BP006248RA
CSB-MP006248RA
CSB-EP006248RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Arachidonate 5-lipoxygenase-activating protein (ALOX5AP), partial CSB-YP001625RB1
CSB-EP001625RB1
CSB-BP001625RB1
CSB-MP001625RB1
CSB-EP001625RB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Muscarinic acetylcholine receptor M2 (CHRM2), partial CSB-YP005382CH1
CSB-EP005382CH1
CSB-BP005382CH1
CSB-MP005382CH1
CSB-EP005382CH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NK-tumor recognition protein (NKTR), partial CSB-YP015838HU
CSB-BP015838HU
CSB-MP015838HU
CSB-EP015838HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Adenylosuccinate synthetase isozyme 2 (ADSS) CSB-YP001398HU
CSB-EP001398HU
CSB-BP001398HU
CSB-MP001398HU
CSB-EP001398HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Alpha-2-macroglobulin receptor-associated protein (LRPAP1) CSB-YP013104HU
CSB-EP013104HU
CSB-BP013104HU
CSB-MP013104HU
CSB-EP013104HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Alpha-2B adrenergic receptor (Adra2b), partial CSB-YP001389MO1
CSB-EP001389MO1
CSB-BP001389MO1
CSB-MP001389MO1
CSB-EP001389MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Proteinase-activated receptor 1 (F2r), partial CSB-YP007924MO1
CSB-EP007924MO1
CSB-BP007924MO1
CSB-MP007924MO1
CSB-EP007924MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human CAP-Gly domain-containing linker protein 1 (CLIP1), partial CSB-YP005553HU
CSB-EP005553HU
CSB-BP005553HU
CSB-MP005553HU
CSB-EP005553HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Probable G-protein coupled receptor 83 (Gpr83), partial CSB-YP009835MO1
CSB-EP009835MO1
CSB-BP009835MO1
CSB-MP009835MO1
CSB-EP009835MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human High affinity cationic amino acid transporter 1 (SLC7A1), partial CSB-YP021709HU
CSB-EP021709HU
CSB-BP021709HU
CSB-MP021709HU
CSB-EP021709HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Fatty aldehyde dehydrogenase (Aldh3a2), partial CSB-YP001573RA1
CSB-EP001573RA1
CSB-BP001573RA1
CSB-MP001573RA1
CSB-EP001573RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Somatostatin receptor type 2 (SSTR2), partial CSB-YP022725HU1
CSB-EP022725HU1
CSB-BP022725HU1
CSB-MP022725HU1
CSB-EP022725HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse C-C motif chemokine 5 (Ccl5) CSB-YP004800MO
CSB-EP004800MO
CSB-BP004800MO
CSB-MP004800MO
CSB-EP004800MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig N (4)- (Beta-N-acetylglucosaminyl)-L-asparaginase (AGA) CSB-YP001423PI
CSB-EP001423PI
CSB-BP001423PI
CSB-MP001423PI
CSB-EP001423PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Neuronal acetylcholine receptor subunit beta-4 (CHRNB4), partial CSB-YP005398HU1
CSB-EP005398HU1
CSB-BP005398HU1
CSB-MP005398HU1
CSB-EP005398HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Heat shock protein HSP 90-alpha (HSP90AA1), partial CSB-YP010802RB
CSB-EP010802RB
CSB-BP010802RB
CSB-MP010802RB
CSB-EP010802RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Olfactory receptor 1E1 (OR1E1), partial CSB-YP016494HU1
CSB-EP016494HU1
CSB-BP016494HU1
CSB-MP016494HU1
CSB-EP016494HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse 5-hydroxytryptamine receptor 5A (Htr5a), partial CSB-YP010896MO1
CSB-EP010896MO1
CSB-BP010896MO1
CSB-MP010896MO1
CSB-EP010896MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Neurotensin receptor type 1 (NTSR1), partial CSB-YP016137HU1
CSB-EP016137HU1
CSB-BP016137HU1
CSB-MP016137HU1
CSB-EP016137HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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