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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Fervidobacterium nodosum Acylphosphatase (acyP) CSB-YP416954FDS
CSB-EP416954FDS
CSB-BP416954FDS
CSB-MP416954FDS
CSB-EP416954FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (Fnod_1307) CSB-YP416955FDS
CSB-EP416955FDS
CSB-BP416955FDS
CSB-MP416955FDS
CSB-EP416955FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Cobalamin synthase (cobS), partial CSB-YP416956FDS1
CSB-EP416956FDS1
CSB-BP416956FDS1
CSB-MP416956FDS1
CSB-EP416956FDS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum SsrA-binding protein (smpB) CSB-YP416957FDS
CSB-EP416957FDS
CSB-BP416957FDS
CSB-MP416957FDS
CSB-EP416957FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum 10 kDa chaperonin (groS) CSB-YP416958FDS
CSB-EP416958FDS
CSB-BP416958FDS
CSB-MP416958FDS
CSB-EP416958FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum UPF0296 protein Fnod_1678 (Fnod_1678) CSB-YP416959FDS
CSB-EP416959FDS
CSB-BP416959FDS
CSB-MP416959FDS
CSB-EP416959FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum Enolase (eno) CSB-YP416960FDS
CSB-EP416960FDS
CSB-BP416960FDS
CSB-MP416960FDS
CSB-EP416960FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fervidobacterium nodosum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP416961FDS
CSB-EP416961FDS
CSB-BP416961FDS
CSB-MP416961FDS
CSB-EP416961FDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans 2-isopropylmalate synthase (leuA), partial CSB-YP416962PYE
CSB-EP416962PYE
CSB-BP416962PYE
CSB-MP416962PYE
CSB-EP416962PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Integration host factor subunit beta (ihfB) CSB-YP416963PYE
CSB-EP416963PYE
CSB-BP416963PYE
CSB-MP416963PYE
CSB-EP416963PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP416964PYE
CSB-EP416964PYE
CSB-BP416964PYE
CSB-MP416964PYE
CSB-EP416964PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Protein ApaG (apaG) CSB-YP416965PYE
CSB-EP416965PYE
CSB-BP416965PYE
CSB-MP416965PYE
CSB-EP416965PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP416966PYE
CSB-EP416966PYE
CSB-BP416966PYE
CSB-MP416966PYE
CSB-EP416966PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Large-conductance mechanosensitive channel (mscL), partial CSB-YP416967PYE1
CSB-EP416967PYE1
CSB-BP416967PYE1
CSB-MP416967PYE1
CSB-EP416967PYE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Potassium-transporting ATPase C chain (kdpC), partial CSB-YP416968PYE1
CSB-EP416968PYE1
CSB-BP416968PYE1
CSB-MP416968PYE1
CSB-EP416968PYE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP416969PYE
CSB-EP416969PYE
CSB-BP416969PYE
CSB-MP416969PYE
CSB-EP416969PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Shikimate dehydrogenase (aroE) CSB-YP416970PYE
CSB-EP416970PYE
CSB-BP416970PYE
CSB-MP416970PYE
CSB-EP416970PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Protease HtpX homolog (htpX), partial CSB-YP416971PYE1
CSB-EP416971PYE1
CSB-BP416971PYE1
CSB-MP416971PYE1
CSB-EP416971PYE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans UPF0386 protein Plav_1374 (Plav_1374) CSB-YP416972PYE
CSB-EP416972PYE
CSB-BP416972PYE
CSB-MP416972PYE
CSB-EP416972PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans 50S ribosomal protein L19 (rplS) CSB-YP416973PYE
CSB-EP416973PYE
CSB-BP416973PYE
CSB-MP416973PYE
CSB-EP416973PYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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