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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Clostridium botulinum Type III pantothenate kinase (coaX) CSB-YP417394CWW
CSB-EP417394CWW
CSB-BP417394CWW
CSB-MP417394CWW
CSB-EP417394CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glutamate racemase (murI) CSB-YP417395CWW
CSB-EP417395CWW
CSB-BP417395CWW
CSB-MP417395CWW
CSB-EP417395CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L9 (rplI) CSB-YP417396CWW
CSB-EP417396CWW
CSB-BP417396CWW
CSB-MP417396CWW
CSB-EP417396CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L34 (rpmH) CSB-YP417397CWW
CSB-EP417397CWW
CSB-BP417397CWW
CSB-MP417397CWW
CSB-EP417397CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP417398BOK
CSB-EP417398BOK
CSB-BP417398BOK
CSB-MP417398BOK
CSB-EP417398BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Bifunctional protein GlmU (glmU) CSB-YP417399BOK
CSB-EP417399BOK
CSB-BP417399BOK
CSB-MP417399BOK
CSB-EP417399BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP417400BOK
CSB-EP417400BOK
CSB-BP417400BOK
CSB-MP417400BOK
CSB-EP417400BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 50S ribosomal protein L7/L12 (rplL) CSB-YP417401BOK
CSB-EP417401BOK
CSB-BP417401BOK
CSB-MP417401BOK
CSB-EP417401BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 50S ribosomal protein L3 (rplC) CSB-YP417402BOK
CSB-EP417402BOK
CSB-BP417402BOK
CSB-MP417402BOK
CSB-EP417402BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 30S ribosomal protein S17 (rpsQ) CSB-YP417403BOK
CSB-EP417403BOK
CSB-BP417403BOK
CSB-MP417403BOK
CSB-EP417403BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 30S ribosomal protein S13 (rpsM) CSB-YP417404BOK
CSB-EP417404BOK
CSB-BP417404BOK
CSB-MP417404BOK
CSB-EP417404BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis D-alanine--D-alanine ligase (ddl) CSB-YP417405BOK
CSB-EP417405BOK
CSB-BP417405BOK
CSB-MP417405BOK
CSB-EP417405BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP417406BOK
CSB-EP417406BOK
CSB-BP417406BOK
CSB-MP417406BOK
CSB-EP417406BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Arginine deiminase (arcA) CSB-YP417407BOK
CSB-EP417407BOK
CSB-BP417407BOK
CSB-MP417407BOK
CSB-EP417407BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Small, acid-soluble spore protein K (sspK) CSB-YP417408BOK
CSB-EP417408BOK
CSB-BP417408BOK
CSB-MP417408BOK
CSB-EP417408BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Probable transaldolase (tal) CSB-YP417409BOK
CSB-EP417409BOK
CSB-BP417409BOK
CSB-MP417409BOK
CSB-EP417409BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis 3'-5' exoribonuclease yhaM (yhaM) CSB-YP417410BOK
CSB-EP417410BOK
CSB-BP417410BOK
CSB-MP417410BOK
CSB-EP417410BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis UPF0736 protein Bcer98_0893 (Bcer98_0893) CSB-YP417411BOK
CSB-EP417411BOK
CSB-BP417411BOK
CSB-MP417411BOK
CSB-EP417411BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP417412BOK
CSB-EP417412BOK
CSB-BP417412BOK
CSB-MP417412BOK
CSB-EP417412BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP417413BOK
CSB-EP417413BOK
CSB-BP417413BOK
CSB-MP417413BOK
CSB-EP417413BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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