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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Replication protein A 70 kDa DNA-binding subunit (RPA1) CSB-YP020088HU
CSB-BP020088HU
CSB-MP020088HU
CSB-EP020088HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein disulfide-isomerase A3 (Pdia3) CSB-YP017720MO
CSB-EP017720MO
CSB-BP017720MO
CSB-MP017720MO
CSB-EP017720MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat cAMP-dependent protein kinase catalytic subunit alpha (Prkaca) CSB-YP018688RA
CSB-EP018688RA
CSB-BP018688RA
CSB-MP018688RA
CSB-EP018688RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Sodium- and chloride-dependent betaine transporter (SLC6A12), partial CSB-YP021690DO
CSB-EP021690DO
CSB-BP021690DO
CSB-MP021690DO
CSB-EP021690DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (Mgat1), partial CSB-YP013773MO1
CSB-EP013773MO1
CSB-BP013773MO1
CSB-MP013773MO1
CSB-EP013773MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Interleukin-1 receptor type 2 (Il1r2), partial CSB-YP011622MO1
CSB-EP011622MO1
CSB-BP011622MO1
CSB-MP011622MO1
CSB-EP011622MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Proteasome subunit beta type-8 (PSMB8) CSB-YP018886HU
CSB-BP018886HU
CSB-MP018886HU
CSB-EP018886HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Proteasome subunit beta type-9 (Psmb9) CSB-YP018887MO
CSB-EP018887MO
CSB-BP018887MO
CSB-MP018887MO
CSB-EP018887MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit C-C motif chemokine 2 (CCL2) CSB-YP004783RB
CSB-EP004783RB
CSB-BP004783RB
CSB-MP004783RB
CSB-EP004783RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Alpha-defensin 5 (Defa5) CSB-YP006657MO
CSB-BP006657MO
CSB-MP006657MO
CSB-EP006657MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Rhodopsin kinase (GRK1), partial CSB-YP009924BO
CSB-EP009924BO
CSB-BP009924BO
CSB-MP009924BO
CSB-EP009924BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse 5-hydroxytryptamine receptor 1B (Htr1b), partial CSB-YP010882MO1
CSB-EP010882MO1
CSB-BP010882MO1
CSB-MP010882MO1
CSB-EP010882MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DNA polymerase delta catalytic subunit (POLD1), partial CSB-YP018303HU
CSB-EP018303HU
CSB-BP018303HU
CSB-MP018303HU
CSB-EP018303HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Homeobox protein MSX-2 (MSX2) CSB-YP015069CH
CSB-EP015069CH
CSB-BP015069CH
CSB-MP015069CH
CSB-EP015069CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Probable global transcription activator SNF2L1 (SMARCA1), partial CSB-YP021798HU
CSB-EP021798HU
CSB-BP021798HU
CSB-MP021798HU
CSB-EP021798HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mitogen-activated protein kinase 1 (MAPK1) CSB-YP013448HU
CSB-EP013448HU
CSB-BP013448HU
CSB-MP013448HU
CSB-EP013448HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Actin-binding protein IPP (Ipp) CSB-YP011782MO
CSB-EP011782MO
CSB-BP011782MO
CSB-MP011782MO
CSB-EP011782MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cytochrome P450 19A1 (Cyp19a1) CSB-YP006394MO
CSB-EP006394MO
CSB-BP006394MO
CSB-MP006394MO
CSB-EP006394MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Nck-associated protein 1 (Nckap1), partial CSB-YP015532MO
CSB-EP015532MO
CSB-BP015532MO
CSB-MP015532MO
CSB-EP015532MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Protein NOV (NOV) CSB-YP015956CH
CSB-EP015956CH
CSB-BP015956CH
CSB-MP015956CH
CSB-EP015956CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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