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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus pumilus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP423437BOL
CSB-EP423437BOL
CSB-BP423437BOL
CSB-MP423437BOL
CSB-EP423437BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Quinolinate synthase A (nadA) CSB-YP423438BOL
CSB-EP423438BOL
CSB-BP423438BOL
CSB-MP423438BOL
CSB-EP423438BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Septum formation protein Maf (maf) CSB-YP423439BOL
CSB-EP423439BOL
CSB-BP423439BOL
CSB-MP423439BOL
CSB-EP423439BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Trigger factor (tig) CSB-YP423440BOL
CSB-EP423440BOL
CSB-BP423440BOL
CSB-MP423440BOL
CSB-EP423440BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Cell division protein ZapA (zapA) CSB-YP423441BOL
CSB-BP423441BOL
CSB-MP423441BOL
CSB-EP423441BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L35 (rpmI) CSB-YP423442BOL
CSB-EP423442BOL
CSB-BP423442BOL
CSB-MP423442BOL
CSB-EP423442BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UPF0354 protein BPUM_2629 (BPUM_2629) CSB-YP423443BOL
CSB-EP423443BOL
CSB-BP423443BOL
CSB-MP423443BOL
CSB-EP423443BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP423444BOL
CSB-EP423444BOL
CSB-BP423444BOL
CSB-MP423444BOL
CSB-EP423444BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Diaminopimelate epimerase (dapF) CSB-YP423445BOL
CSB-EP423445BOL
CSB-BP423445BOL
CSB-MP423445BOL
CSB-EP423445BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Uronate isomerase (uxaC) CSB-YP423446BOL
CSB-EP423446BOL
CSB-BP423446BOL
CSB-MP423446BOL
CSB-EP423446BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP423447BOL
CSB-EP423447BOL
CSB-BP423447BOL
CSB-MP423447BOL
CSB-EP423447BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP423448BOL
CSB-EP423448BOL
CSB-BP423448BOL
CSB-MP423448BOL
CSB-EP423448BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Flagellar assembly factor FliW (fliW) CSB-YP423449BOL
CSB-EP423449BOL
CSB-BP423449BOL
CSB-MP423449BOL
CSB-EP423449BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus D-ribose pyranase (rbsD) CSB-YP423450BOL
CSB-EP423450BOL
CSB-BP423450BOL
CSB-MP423450BOL
CSB-EP423450BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus UV DNA damage endonuclease (uvsE) CSB-YP423451BOL
CSB-EP423451BOL
CSB-BP423451BOL
CSB-MP423451BOL
CSB-EP423451BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Peptidase T (pepT) CSB-YP423452BOL
CSB-EP423452BOL
CSB-BP423452BOL
CSB-MP423452BOL
CSB-EP423452BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Adenylosuccinate synthetase (purA) CSB-YP423453BOL
CSB-EP423453BOL
CSB-BP423453BOL
CSB-MP423453BOL
CSB-EP423453BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Nucleoid occlusion protein (noc) CSB-YP423454BOL
CSB-EP423454BOL
CSB-BP423454BOL
CSB-MP423454BOL
CSB-EP423454BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus 50S ribosomal protein L34 (rpmH) CSB-YP423455BOL
CSB-EP423455BOL
CSB-BP423455BOL
CSB-MP423455BOL
CSB-EP423455BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Dihydroxy-acid dehydratase (ilvD), partial CSB-YP423456CWG
CSB-EP423456CWG
CSB-BP423456CWG
CSB-MP423456CWG
CSB-EP423456CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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