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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella sediminis Ribonuclease H (rnhA) CSB-YP423517STR
CSB-EP423517STR
CSB-BP423517STR
CSB-MP423517STR
CSB-EP423517STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP423518STR
CSB-EP423518STR
CSB-BP423518STR
CSB-MP423518STR
CSB-EP423518STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 50S ribosomal protein L35 (rpmI) CSB-YP423519STR
CSB-EP423519STR
CSB-BP423519STR
CSB-MP423519STR
CSB-EP423519STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP423520STR
CSB-EP423520STR
CSB-BP423520STR
CSB-MP423520STR
CSB-EP423520STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Elongation factor P (efp) CSB-YP423521STR
CSB-EP423521STR
CSB-BP423521STR
CSB-MP423521STR
CSB-EP423521STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 50S ribosomal protein L25 (rplY) CSB-YP423522STR
CSB-EP423522STR
CSB-BP423522STR
CSB-MP423522STR
CSB-EP423522STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Probable septum site-determining protein MinC (minC) CSB-YP423523STR
CSB-EP423523STR
CSB-BP423523STR
CSB-MP423523STR
CSB-EP423523STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 50S ribosomal protein L32 (rpmF) CSB-YP423524STR
CSB-EP423524STR
CSB-BP423524STR
CSB-MP423524STR
CSB-EP423524STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis N-succinylarginine dihydrolase (astB) CSB-YP423525STR
CSB-EP423525STR
CSB-BP423525STR
CSB-MP423525STR
CSB-EP423525STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Glutamate--tRNA ligase (gltX) CSB-YP423526STR
CSB-EP423526STR
CSB-BP423526STR
CSB-MP423526STR
CSB-EP423526STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Ribonuclease HII (rnhB) CSB-YP423527STR
CSB-EP423527STR
CSB-BP423527STR
CSB-MP423527STR
CSB-EP423527STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis tRNA sulfurtransferase (thiI) CSB-YP423528STR
CSB-EP423528STR
CSB-BP423528STR
CSB-MP423528STR
CSB-EP423528STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Deoxyribose-phosphate aldolase (deoC) CSB-YP423529STR
CSB-EP423529STR
CSB-BP423529STR
CSB-MP423529STR
CSB-EP423529STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis DNA polymerase IV (dinB) CSB-YP423530STR
CSB-EP423530STR
CSB-BP423530STR
CSB-MP423530STR
CSB-EP423530STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis UPF0234 protein Ssed_3443 (Ssed_3443) CSB-YP423531STR
CSB-EP423531STR
CSB-BP423531STR
CSB-MP423531STR
CSB-EP423531STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Peptidyl-tRNA hydrolase (pth) CSB-YP423532STR
CSB-EP423532STR
CSB-BP423532STR
CSB-MP423532STR
CSB-EP423532STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP423533STR
CSB-EP423533STR
CSB-BP423533STR
CSB-MP423533STR
CSB-EP423533STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Tryptophanase (tnaA) CSB-YP423534STR
CSB-EP423534STR
CSB-BP423534STR
CSB-MP423534STR
CSB-EP423534STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Regulator of ribonuclease activity A (rraA) CSB-YP423535STR
CSB-EP423535STR
CSB-BP423535STR
CSB-MP423535STR
CSB-EP423535STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Ribosomal protein L11 methyltransferase (prmA) CSB-YP423536STR
CSB-EP423536STR
CSB-BP423536STR
CSB-MP423536STR
CSB-EP423536STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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