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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus gordonii 30S ribosomal protein S13 (rpsM) CSB-YP424317FMN
CSB-EP424317FMN
CSB-BP424317FMN
CSB-MP424317FMN
CSB-EP424317FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 50S ribosomal protein L5 (rplE) CSB-YP424318FMN
CSB-EP424318FMN
CSB-BP424318FMN
CSB-MP424318FMN
CSB-EP424318FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 50S ribosomal protein L22 (rplV) CSB-YP424319FMN
CSB-EP424319FMN
CSB-BP424319FMN
CSB-MP424319FMN
CSB-EP424319FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP424320FMN
CSB-EP424320FMN
CSB-BP424320FMN
CSB-MP424320FMN
CSB-EP424320FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Peptidyl-tRNA hydrolase (pth) CSB-YP424321FMN
CSB-EP424321FMN
CSB-BP424321FMN
CSB-MP424321FMN
CSB-EP424321FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis ATPase ASNA1 homolog (GL50803_7953) CSB-YP424322GFY
CSB-EP424322GFY
CSB-BP424322GFY
CSB-MP424322GFY
CSB-EP424322GFY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP424323ILV
CSB-EP424323ILV
CSB-BP424323ILV
CSB-MP424323ILV
CSB-EP424323ILV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 2 (M2), partial CSB-YP424324ILP1
CSB-EP424324ILP1
CSB-BP424324ILP1
CSB-MP424324ILP1
CSB-EP424324ILP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizophora stylosa Germanicol synthase (M1), partial CSB-YP424325RII
CSB-EP424325RII
CSB-BP424325RII
CSB-MP424325RII
CSB-EP424325RII-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proline racemase CSB-YP424326DUN
CSB-EP424326DUN
CSB-BP424326DUN
CSB-MP424326DUN
CSB-EP424326DUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Putative RNA polymerase II subunit B1 CTD phosphatase RPAP2 homolog (CG34183) CSB-YP424327DLU
CSB-EP424327DLU
CSB-BP424327DLU
CSB-MP424327DLU
CSB-EP424327DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis N-alpha-acetyltransferase 60 (naa60) CSB-YP424328XBF
CSB-EP424328XBF
CSB-BP424328XBF
CSB-MP424328XBF
CSB-EP424328XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cytochrome c oxidase assembly protein COX14 homolog (si:dkey-222f2.8), partial CSB-YP424329DIL1
CSB-EP424329DIL1
CSB-BP424329DIL1
CSB-MP424329DIL1
CSB-EP424329DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Glutamate--tRNA ligase 1 (gltX1) CSB-YP424330AUY
CSB-EP424330AUY
CSB-BP424330AUY
CSB-MP424330AUY
CSB-EP424330AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Phosphoglucosamine mutase (glmM) CSB-YP424331AUY
CSB-EP424331AUY
CSB-BP424331AUY
CSB-MP424331AUY
CSB-EP424331AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri 30S ribosomal protein S9 (rpsI) CSB-YP424332AUY
CSB-EP424332AUY
CSB-BP424332AUY
CSB-MP424332AUY
CSB-EP424332AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Adenylosuccinate synthetase (purA) CSB-YP424333AUY
CSB-EP424333AUY
CSB-BP424333AUY
CSB-MP424333AUY
CSB-EP424333AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP424334AUY
CSB-EP424334AUY
CSB-BP424334AUY
CSB-MP424334AUY
CSB-EP424334AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri UPF0102 protein Abu_0255 (Abu_0255) CSB-YP424335AUY
CSB-EP424335AUY
CSB-BP424335AUY
CSB-MP424335AUY
CSB-EP424335AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP424336AUY
CSB-EP424336AUY
CSB-BP424336AUY
CSB-MP424336AUY
CSB-EP424336AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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