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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Citrobacter koseri Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP425319CWS
CSB-EP425319CWS
CSB-BP425319CWS
CSB-MP425319CWS
CSB-EP425319CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP425320CWS
CSB-EP425320CWS
CSB-BP425320CWS
CSB-MP425320CWS
CSB-EP425320CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii DNA replication and repair protein recF (recF) CSB-YP425321FMN
CSB-EP425321FMN
CSB-BP425321FMN
CSB-MP425321FMN
CSB-EP425321FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP425322FMN
CSB-EP425322FMN
CSB-BP425322FMN
CSB-MP425322FMN
CSB-EP425322FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP425323FMN
CSB-EP425323FMN
CSB-BP425323FMN
CSB-MP425323FMN
CSB-EP425323FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Protein translocase subunit SecA (secA), partial CSB-YP425324FMN
CSB-EP425324FMN
CSB-BP425324FMN
CSB-MP425324FMN
CSB-EP425324FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii UPF0348 protein SGO_0452 (SGO_0452) CSB-YP425325FMN
CSB-EP425325FMN
CSB-BP425325FMN
CSB-MP425325FMN
CSB-EP425325FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Transcription elongation factor GreA (greA) CSB-YP425326FMN
CSB-EP425326FMN
CSB-BP425326FMN
CSB-MP425326FMN
CSB-EP425326FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Holliday junction resolvase recU (recU) CSB-YP425327FMN
CSB-EP425327FMN
CSB-BP425327FMN
CSB-MP425327FMN
CSB-EP425327FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP425328FMN
CSB-EP425328FMN
CSB-BP425328FMN
CSB-MP425328FMN
CSB-EP425328FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Cell division protein DivIB (divIB), partial CSB-YP425329FMN
CSB-EP425329FMN
CSB-BP425329FMN
CSB-MP425329FMN
CSB-EP425329FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP425330FMN
CSB-EP425330FMN
CSB-BP425330FMN
CSB-MP425330FMN
CSB-EP425330FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Threonine--tRNA ligase (thrS), partial CSB-YP425331FMN
CSB-EP425331FMN
CSB-BP425331FMN
CSB-MP425331FMN
CSB-EP425331FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Putative phosphotransferase SGO_0812 (SGO_0812) CSB-YP425332FMN
CSB-EP425332FMN
CSB-BP425332FMN
CSB-MP425332FMN
CSB-EP425332FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii GTPase obg (obg) CSB-YP425333FMN
CSB-EP425333FMN
CSB-BP425333FMN
CSB-MP425333FMN
CSB-EP425333FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Homoserine O-succinyltransferase (metA) CSB-YP425334FMN
CSB-EP425334FMN
CSB-BP425334FMN
CSB-MP425334FMN
CSB-EP425334FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Bifunctional protein pyrR (pyrR) CSB-YP425335FMN
CSB-EP425335FMN
CSB-BP425335FMN
CSB-MP425335FMN
CSB-EP425335FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Probable GTP-binding protein EngB (engB) CSB-YP425336FMN
CSB-EP425336FMN
CSB-BP425336FMN
CSB-MP425336FMN
CSB-EP425336FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 50S ribosomal protein L7/L12 (rplL) CSB-YP425337FMN
CSB-EP425337FMN
CSB-BP425337FMN
CSB-MP425337FMN
CSB-EP425337FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii UPF0758 protein SGO_1229 (SGO_1229) CSB-YP425338FMN
CSB-EP425338FMN
CSB-BP425338FMN
CSB-MP425338FMN
CSB-EP425338FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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