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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dinoroseobacter shibae DNA repair protein recO (recO) CSB-YP424919DIB
CSB-EP424919DIB
CSB-BP424919DIB
CSB-MP424919DIB
CSB-EP424919DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP424920DIB
CSB-EP424920DIB
CSB-BP424920DIB
CSB-MP424920DIB
CSB-EP424920DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae 50S ribosomal protein L10 (rplJ) CSB-YP424921DIB
CSB-EP424921DIB
CSB-BP424921DIB
CSB-MP424921DIB
CSB-EP424921DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Integration host factor subunit alpha (ihfA) CSB-YP424922DIB
CSB-EP424922DIB
CSB-BP424922DIB
CSB-MP424922DIB
CSB-EP424922DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP424923DIB
CSB-EP424923DIB
CSB-BP424923DIB
CSB-MP424923DIB
CSB-EP424923DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae 50S ribosomal protein L4 (rplD) CSB-YP424924DIB
CSB-EP424924DIB
CSB-BP424924DIB
CSB-MP424924DIB
CSB-EP424924DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae 30S ribosomal protein S3 (rpsC) CSB-YP424925DIB
CSB-EP424925DIB
CSB-BP424925DIB
CSB-MP424925DIB
CSB-EP424925DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae 30S ribosomal protein S5 (rpsE) CSB-YP424926DIB
CSB-EP424926DIB
CSB-BP424926DIB
CSB-MP424926DIB
CSB-EP424926DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Putative membrane protein insertion efficiency factor (Dshi_0331) CSB-YP424927DIB
CSB-EP424927DIB
CSB-BP424927DIB
CSB-MP424927DIB
CSB-EP424927DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Carboxylate-amine ligase Dshi_1749 (Dshi_1749) CSB-YP424928DIB
CSB-EP424928DIB
CSB-BP424928DIB
CSB-MP424928DIB
CSB-EP424928DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Chorismate synthase (aroC) CSB-YP424929DIB
CSB-EP424929DIB
CSB-BP424929DIB
CSB-MP424929DIB
CSB-EP424929DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP424930DIB
CSB-EP424930DIB
CSB-BP424930DIB
CSB-MP424930DIB
CSB-EP424930DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Xylose isomerase (xylA) CSB-YP424931DIB
CSB-EP424931DIB
CSB-BP424931DIB
CSB-MP424931DIB
CSB-EP424931DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP424932DIB
CSB-EP424932DIB
CSB-BP424932DIB
CSB-MP424932DIB
CSB-EP424932DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Protein translocase subunit SecA (secA), partial CSB-YP424933DIB
CSB-EP424933DIB
CSB-BP424933DIB
CSB-MP424933DIB
CSB-EP424933DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP424934DIB
CSB-EP424934DIB
CSB-BP424934DIB
CSB-MP424934DIB
CSB-EP424934DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae 50S ribosomal protein L9 (rplI) CSB-YP424935DIB
CSB-EP424935DIB
CSB-BP424935DIB
CSB-MP424935DIB
CSB-EP424935DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Threonine--tRNA ligase (thrS), partial CSB-YP424936DIB
CSB-EP424936DIB
CSB-BP424936DIB
CSB-MP424936DIB
CSB-EP424936DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Urease accessory protein UreG (ureG) CSB-YP424937DIB
CSB-EP424937DIB
CSB-BP424937DIB
CSB-MP424937DIB
CSB-EP424937DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Ketol-acid reductoisomerase (ilvC) CSB-YP424938DIB
CSB-EP424938DIB
CSB-BP424938DIB
CSB-MP424938DIB
CSB-EP424938DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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