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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O139:H28 Autoinducer 2 kinase LsrK (lsrK) CSB-YP424037EJD
CSB-EP424037EJD
CSB-BP424037EJD
CSB-MP424037EJD
CSB-EP424037EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0056 inner membrane protein marC (marC), partial CSB-YP424038EJD1
CSB-EP424038EJD1
CSB-BP424038EJD1
CSB-MP424038EJD1
CSB-EP424038EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Dihydrofolate reductase folM (folM) CSB-YP424039EJD
CSB-EP424039EJD
CSB-BP424039EJD
CSB-MP424039EJD
CSB-EP424039EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP424040EJD
CSB-EP424040EJD
CSB-BP424040EJD
CSB-MP424040EJD
CSB-EP424040EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Integration host factor subunit alpha (ihfA) CSB-YP424041EJD
CSB-EP424041EJD
CSB-BP424041EJD
CSB-MP424041EJD
CSB-EP424041EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Succinylglutamate desuccinylase (astE) CSB-YP424042EJD
CSB-EP424042EJD
CSB-BP424042EJD
CSB-MP424042EJD
CSB-EP424042EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0756 membrane protein YeaL (yeaL) CSB-YP424043EJD
CSB-EP424043EJD
CSB-BP424043EJD
CSB-MP424043EJD
CSB-EP424043EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Protease HtpX (htpX), partial CSB-YP424044EJD1
CSB-EP424044EJD1
CSB-BP424044EJD1
CSB-MP424044EJD1
CSB-EP424044EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 tRNA (mo5U34)-methyltransferase CSB-YP424045EJD
CSB-EP424045EJD
CSB-BP424045EJD
CSB-MP424045EJD
CSB-EP424045EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Flagellar protein FliT (fliT) CSB-YP424046EJD
CSB-EP424046EJD
CSB-BP424046EJD
CSB-MP424046EJD
CSB-EP424046EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Cobalamin synthase (cobS), partial CSB-YP424047EJD1
CSB-EP424047EJD1
CSB-BP424047EJD1
CSB-MP424047EJD1
CSB-EP424047EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Multidrug resistance protein MdtB (mdtB), partial CSB-YP424048EJD
CSB-EP424048EJD
CSB-BP424048EJD
CSB-MP424048EJD
CSB-EP424048EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Methionine--tRNA ligase (metG) CSB-YP424049EJD
CSB-EP424049EJD
CSB-BP424049EJD
CSB-MP424049EJD
CSB-EP424049EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Elongation factor P-like protein (yeiP) CSB-YP424050EJD
CSB-EP424050EJD
CSB-BP424050EJD
CSB-MP424050EJD
CSB-EP424050EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP424051EJD
CSB-EP424051EJD
CSB-BP424051EJD
CSB-MP424051EJD
CSB-EP424051EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP424052EJD
CSB-EP424052EJD
CSB-BP424052EJD
CSB-MP424052EJD
CSB-EP424052EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP424053EJD
CSB-EP424053EJD
CSB-BP424053EJD
CSB-MP424053EJD
CSB-EP424053EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UPF0208 membrane protein YfbV (yfbV), partial CSB-YP424054EJD1
CSB-EP424054EJD1
CSB-BP424054EJD1
CSB-MP424054EJD1
CSB-EP424054EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Fatty acid oxidation complex subunit alpha (fadJ) CSB-YP424055EJD
CSB-EP424055EJD
CSB-BP424055EJD
CSB-MP424055EJD
CSB-EP424055EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 DNA ligase (ligA) CSB-YP424056EJD
CSB-EP424056EJD
CSB-BP424056EJD
CSB-MP424056EJD
CSB-EP424056EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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