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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus amyloliquefaciens Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP423977BQD
CSB-EP423977BQD
CSB-BP423977BQD
CSB-MP423977BQD
CSB-EP423977BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Flagellar protein FliT (fliT) CSB-YP423978BQD
CSB-EP423978BQD
CSB-BP423978BQD
CSB-MP423978BQD
CSB-EP423978BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP423979BQD
CSB-EP423979BQD
CSB-BP423979BQD
CSB-MP423979BQD
CSB-EP423979BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Hydroxyethylthiazole kinase (thiM) CSB-YP423980BQD
CSB-EP423980BQD
CSB-BP423980BQD
CSB-MP423980BQD
CSB-EP423980BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 5-dehydro-2-deoxygluconokinase (iolC) CSB-YP423981BQD
CSB-EP423981BQD
CSB-BP423981BQD
CSB-MP423981BQD
CSB-EP423981BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Probable manganese-dependent inorganic pyrophosphatase (ppaC) CSB-YP423982BQD
CSB-EP423982BQD
CSB-BP423982BQD
CSB-MP423982BQD
CSB-EP423982BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 50S ribosomal protein L34 (rpmH) CSB-YP423983BQD
CSB-EP423983BQD
CSB-BP423983BQD
CSB-MP423983BQD
CSB-EP423983BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Phosphoglucosamine mutase (glmM) CSB-YP423984CWA
CSB-EP423984CWA
CSB-BP423984CWA
CSB-MP423984CWA
CSB-EP423984CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus UPF0102 protein Ccon26_01140 (Ccon26_01140) CSB-YP423985CWA
CSB-EP423985CWA
CSB-BP423985CWA
CSB-MP423985CWA
CSB-EP423985CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Na (+)/H (+) antiporter nhaA, partial CSB-YP423986CWA
CSB-EP423986CWA
CSB-BP423986CWA
CSB-MP423986CWA
CSB-EP423986CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus N utilization substance protein B homolog (nusB) CSB-YP423987CWA
CSB-EP423987CWA
CSB-BP423987CWA
CSB-MP423987CWA
CSB-EP423987CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP423988CWA
CSB-EP423988CWA
CSB-BP423988CWA
CSB-MP423988CWA
CSB-EP423988CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Large-conductance mechanosensitive channel (mscL), partial CSB-YP423989CWA1
CSB-EP423989CWA1
CSB-BP423989CWA1
CSB-MP423989CWA1
CSB-EP423989CWA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Bifunctional protein FolD (folD) CSB-YP423990CWA
CSB-EP423990CWA
CSB-BP423990CWA
CSB-MP423990CWA
CSB-EP423990CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus UPF0763 protein Ccon26_06450 (Ccon26_06450) CSB-YP423991CWA
CSB-EP423991CWA
CSB-BP423991CWA
CSB-MP423991CWA
CSB-EP423991CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus 50S ribosomal protein L7/L12 (rplL) CSB-YP423992CWA
CSB-EP423992CWA
CSB-BP423992CWA
CSB-MP423992CWA
CSB-EP423992CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus 50S ribosomal protein L34 (rpmH) CSB-YP423993CWA
CSB-EP423993CWA
CSB-BP423993CWA
CSB-MP423993CWA
CSB-EP423993CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus 50S ribosomal protein L9 (rplI) CSB-YP423994CWA
CSB-EP423994CWA
CSB-BP423994CWA
CSB-MP423994CWA
CSB-EP423994CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Histidine--tRNA ligase (hisS) CSB-YP423995CWA
CSB-EP423995CWA
CSB-BP423995CWA
CSB-MP423995CWA
CSB-EP423995CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus tRNA (uracil (54)-C (5))-methyltransferase CSB-YP423996CWA
CSB-EP423996CWA
CSB-BP423996CWA
CSB-MP423996CWA
CSB-EP423996CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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