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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Campylobacter concisus Phosphoheptose isomerase (gmhA) CSB-YP423997CWA
CSB-EP423997CWA
CSB-BP423997CWA
CSB-MP423997CWA
CSB-EP423997CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Alanine--tRNA ligase (alaS), partial CSB-YP423998CWA
CSB-EP423998CWA
CSB-BP423998CWA
CSB-MP423998CWA
CSB-EP423998CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Ketol-acid reductoisomerase (ilvC) CSB-YP423999CWA
CSB-EP423999CWA
CSB-BP423999CWA
CSB-MP423999CWA
CSB-EP423999CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP424000CWA
CSB-EP424000CWA
CSB-BP424000CWA
CSB-MP424000CWA
CSB-EP424000CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP424001CWA
CSB-EP424001CWA
CSB-BP424001CWA
CSB-MP424001CWA
CSB-EP424001CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus ATP phosphoribosyltransferase (hisG) CSB-YP424002CWA
CSB-EP424002CWA
CSB-BP424002CWA
CSB-MP424002CWA
CSB-EP424002CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Enolase (eno) CSB-YP424003CWA
CSB-EP424003CWA
CSB-BP424003CWA
CSB-MP424003CWA
CSB-EP424003CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus 50S ribosomal protein L17 (rplQ) CSB-YP424004CWA
CSB-EP424004CWA
CSB-BP424004CWA
CSB-MP424004CWA
CSB-EP424004CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus 30S ribosomal protein S11 (rpsK) CSB-YP424005CWA
CSB-EP424005CWA
CSB-BP424005CWA
CSB-MP424005CWA
CSB-EP424005CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus 50S ribosomal protein L5 (rplE) CSB-YP424006CWA
CSB-EP424006CWA
CSB-BP424006CWA
CSB-MP424006CWA
CSB-EP424006CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus 50S ribosomal protein L2 (rplB) CSB-YP424007CWA
CSB-EP424007CWA
CSB-BP424007CWA
CSB-MP424007CWA
CSB-EP424007CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Orotate phosphoribosyltransferase (pyrE) CSB-YP424008CWA
CSB-EP424008CWA
CSB-BP424008CWA
CSB-MP424008CWA
CSB-EP424008CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Na (+)/H (+) antiporter nhaA, partial CSB-YP424009EJD
CSB-EP424009EJD
CSB-BP424009EJD
CSB-MP424009EJD
CSB-EP424009EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Carnitine operon protein CaiE (caiE) CSB-YP424010EJD
CSB-EP424010EJD
CSB-BP424010EJD
CSB-MP424010EJD
CSB-EP424010EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Glutathione-regulated potassium-efflux system ancillary protein kefF (kefF) CSB-YP424011EJD
CSB-EP424011EJD
CSB-BP424011EJD
CSB-MP424011EJD
CSB-EP424011EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Ribulokinase (araB) CSB-YP424012EJD
CSB-EP424012EJD
CSB-BP424012EJD
CSB-MP424012EJD
CSB-EP424012EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP424013EJD
CSB-EP424013EJD
CSB-BP424013EJD
CSB-MP424013EJD
CSB-EP424013EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP424014EJD
CSB-EP424014EJD
CSB-BP424014EJD
CSB-MP424014EJD
CSB-EP424014EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP424015EJD
CSB-EP424015EJD
CSB-BP424015EJD
CSB-MP424015EJD
CSB-EP424015EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Xanthine phosphoribosyltransferase (gpt) CSB-YP424016EJD
CSB-EP424016EJD
CSB-BP424016EJD
CSB-MP424016EJD
CSB-EP424016EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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