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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O9:H4 Ribonuclease P protein component (rnpA) CSB-YP424177EJF
CSB-EP424177EJF
CSB-BP424177EJF
CSB-MP424177EJF
CSB-EP424177EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Inner membrane protein CbrB (cbrB), partial CSB-YP424178EJF1
CSB-EP424178EJF1
CSB-BP424178EJF1
CSB-MP424178EJF1
CSB-EP424178EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 4-alpha-L-fucosyltransferase (wecF) CSB-YP424179EJF
CSB-EP424179EJF
CSB-BP424179EJF
CSB-MP424179EJF
CSB-EP424179EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP424180EJF1
CSB-EP424180EJF1
CSB-BP424180EJF1
CSB-MP424180EJF1
CSB-EP424180EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Protein FdhE (fdhE) CSB-YP424181EJF
CSB-EP424181EJF
CSB-BP424181EJF
CSB-MP424181EJF
CSB-EP424181EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Ferrous-iron efflux pump FieF (fieF), partial CSB-YP424182EJF1
CSB-EP424182EJF1
CSB-BP424182EJF1
CSB-MP424182EJF1
CSB-EP424182EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Regulator of ribonuclease activity A (rraA) CSB-YP424183EJF
CSB-EP424183EJF
CSB-BP424183EJF
CSB-MP424183EJF
CSB-EP424183EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Acetylornithine deacetylase (argE) CSB-YP424184EJF
CSB-EP424184EJF
CSB-BP424184EJF
CSB-MP424184EJF
CSB-EP424184EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Glutamate racemase (murI) CSB-YP424185EJF
CSB-EP424185EJF
CSB-BP424185EJF
CSB-MP424185EJF
CSB-EP424185EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Thiamine-phosphate synthase (thiE) CSB-YP424186EJF
CSB-EP424186EJF
CSB-BP424186EJF
CSB-MP424186EJF
CSB-EP424186EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Protein psiE (psiE), partial CSB-YP424187EJF1
CSB-EP424187EJF1
CSB-BP424187EJF1
CSB-MP424187EJF1
CSB-EP424187EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Divalent-cation tolerance protein CutA (cutA) CSB-YP424188EJF
CSB-EP424188EJF
CSB-BP424188EJF
CSB-MP424188EJF
CSB-EP424188EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Fumarate reductase subunit D (frdD), partial CSB-YP424189EJF1
CSB-EP424189EJF1
CSB-BP424189EJF1
CSB-MP424189EJF1
CSB-EP424189EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 L-ribulose-5-phosphate 4-epimerase UlaF (ulaF) CSB-YP424190EJF
CSB-EP424190EJF
CSB-BP424190EJF
CSB-MP424190EJF
CSB-EP424190EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 MgtA leader peptide (mgtL) CSB-YP424191EJF
CSB-EP424191EJF
CSB-BP424191EJF
CSB-MP424191EJF
CSB-EP424191EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 N-acetylneuraminate epimerase (nanM) CSB-YP424192EJF
CSB-EP424192EJF
CSB-BP424192EJF
CSB-MP424192EJF
CSB-EP424192EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Deoxyribose-phosphate aldolase (deoC) CSB-YP424193EJF
CSB-EP424193EJF
CSB-BP424193EJF
CSB-MP424193EJF
CSB-EP424193EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Aspartate carbamoyltransferase (pyrB) CSB-YP424194ILH
CSB-EP424194ILH
CSB-BP424194ILH
CSB-MP424194ILH
CSB-EP424194ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP424195ILH
CSB-EP424195ILH
CSB-BP424195ILH
CSB-MP424195ILH
CSB-EP424195ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ignicoccus hospitalis Shikimate dehydrogenase (aroE) CSB-YP424196ILH
CSB-EP424196ILH
CSB-BP424196ILH
CSB-MP424196ILH
CSB-EP424196ILH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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