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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arcobacter butzleri 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) CSB-YP425380AUY
CSB-EP425380AUY
CSB-BP425380AUY
CSB-MP425380AUY
CSB-EP425380AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Homoserine kinase (thrB) CSB-YP425381AUY
CSB-EP425381AUY
CSB-BP425381AUY
CSB-MP425381AUY
CSB-EP425381AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Arginine--tRNA ligase (argS), partial CSB-YP425382AUY
CSB-EP425382AUY
CSB-BP425382AUY
CSB-MP425382AUY
CSB-EP425382AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Protein grpE (grpE) CSB-YP425383AUY
CSB-EP425383AUY
CSB-BP425383AUY
CSB-MP425383AUY
CSB-EP425383AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP425384AUY
CSB-EP425384AUY
CSB-BP425384AUY
CSB-MP425384AUY
CSB-EP425384AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP425385RMR
CSB-EP425385RMR
CSB-BP425385RMR
CSB-MP425385RMR
CSB-EP425385RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 30S ribosomal protein S18 (rpsR) CSB-YP425386RMR
CSB-EP425386RMR
CSB-BP425386RMR
CSB-MP425386RMR
CSB-EP425386RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Deoxycytidine triphosphate deaminase (dcd) CSB-YP425387RMR
CSB-EP425387RMR
CSB-BP425387RMR
CSB-MP425387RMR
CSB-EP425387RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 50S ribosomal protein L19 (rplS) CSB-YP425388RMR
CSB-EP425388RMR
CSB-BP425388RMR
CSB-MP425388RMR
CSB-EP425388RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 30S ribosomal protein S12 (rpsL) CSB-YP425389RMR
CSB-EP425389RMR
CSB-BP425389RMR
CSB-MP425389RMR
CSB-EP425389RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Aspartate--tRNA ligase (aspS), partial CSB-YP425390RMR
CSB-EP425390RMR
CSB-BP425390RMR
CSB-MP425390RMR
CSB-EP425390RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Chaperone protein DnaJ (dnaJ) CSB-YP425391RMR
CSB-EP425391RMR
CSB-BP425391RMR
CSB-MP425391RMR
CSB-EP425391RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP425392RMR
CSB-EP425392RMR
CSB-BP425392RMR
CSB-MP425392RMR
CSB-EP425392RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis RNA pyrophosphohydrolase (rppH) CSB-YP425393RMR
CSB-EP425393RMR
CSB-BP425393RMR
CSB-MP425393RMR
CSB-EP425393RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Ribonuclease PH (rph) CSB-YP425394RMR
CSB-EP425394RMR
CSB-BP425394RMR
CSB-MP425394RMR
CSB-EP425394RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis 30S ribosomal protein S21 (rpsU) CSB-YP425395RMR
CSB-EP425395RMR
CSB-BP425395RMR
CSB-MP425395RMR
CSB-EP425395RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP425396RMR
CSB-EP425396RMR
CSB-BP425396RMR
CSB-MP425396RMR
CSB-EP425396RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis DNA repair protein recO (recO) CSB-YP425397RMR
CSB-EP425397RMR
CSB-BP425397RMR
CSB-MP425397RMR
CSB-EP425397RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Probable transcriptional regulatory protein A1E_02520 (A1E_02520) CSB-YP425398RMR
CSB-EP425398RMR
CSB-BP425398RMR
CSB-MP425398RMR
CSB-EP425398RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis tRNA pseudouridine synthase B (truB) CSB-YP425399RMR
CSB-EP425399RMR
CSB-BP425399RMR
CSB-MP425399RMR
CSB-EP425399RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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