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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Citrobacter koseri 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP424237CWS
CSB-EP424237CWS
CSB-BP424237CWS
CSB-MP424237CWS
CSB-EP424237CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Serine--tRNA ligase (serS) CSB-YP424238CWS
CSB-EP424238CWS
CSB-BP424238CWS
CSB-MP424238CWS
CSB-EP424238CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP424239CWS
CSB-EP424239CWS
CSB-BP424239CWS
CSB-MP424239CWS
CSB-EP424239CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Glutamine--tRNA ligase (glnS), partial CSB-YP424240CWS
CSB-EP424240CWS
CSB-BP424240CWS
CSB-MP424240CWS
CSB-EP424240CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Octanoyltransferase (lipB) CSB-YP424241CWS
CSB-EP424241CWS
CSB-BP424241CWS
CSB-MP424241CWS
CSB-EP424241CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Probable 2- (5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase CSB-YP424242CWS
CSB-EP424242CWS
CSB-BP424242CWS
CSB-MP424242CWS
CSB-EP424242CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Chaperone protein htpG (htpG), partial CSB-YP424243CWS
CSB-EP424243CWS
CSB-BP424243CWS
CSB-MP424243CWS
CSB-EP424243CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 7-cyano-7-deazaguanine synthase (queC) CSB-YP424244CWS
CSB-EP424244CWS
CSB-BP424244CWS
CSB-MP424244CWS
CSB-EP424244CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP424245CWS
CSB-EP424245CWS
CSB-BP424245CWS
CSB-MP424245CWS
CSB-EP424245CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Gamma-glutamyl phosphate reductase (proA) CSB-YP424246CWS
CSB-EP424246CWS
CSB-BP424246CWS
CSB-MP424246CWS
CSB-EP424246CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Endonuclease V (nfi) CSB-YP424247CWS
CSB-EP424247CWS
CSB-BP424247CWS
CSB-MP424247CWS
CSB-EP424247CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 50S ribosomal protein L7/L12 (rplL) CSB-YP424248CWS
CSB-EP424248CWS
CSB-BP424248CWS
CSB-MP424248CWS
CSB-EP424248CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP424249CWS
CSB-EP424249CWS
CSB-BP424249CWS
CSB-MP424249CWS
CSB-EP424249CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Cell division protein ZapB (zapB) CSB-YP424250CWS
CSB-EP424250CWS
CSB-BP424250CWS
CSB-MP424250CWS
CSB-EP424250CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri L-rhamnose isomerase (rhaA) CSB-YP424251CWS
CSB-EP424251CWS
CSB-BP424251CWS
CSB-MP424251CWS
CSB-EP424251CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Ribonuclease HII (rnhB) CSB-YP424252CWS
CSB-EP424252CWS
CSB-BP424252CWS
CSB-MP424252CWS
CSB-EP424252CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri 30S ribosomal protein S2 (rpsB) CSB-YP424253CWS
CSB-EP424253CWS
CSB-BP424253CWS
CSB-MP424253CWS
CSB-EP424253CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Sugar fermentation stimulation protein homolog (sfsA) CSB-YP424254CWS
CSB-EP424254CWS
CSB-BP424254CWS
CSB-MP424254CWS
CSB-EP424254CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri GMP reductase (guaC) CSB-YP424255CWS
CSB-EP424255CWS
CSB-BP424255CWS
CSB-MP424255CWS
CSB-EP424255CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP424256CWS
CSB-EP424256CWS
CSB-BP424256CWS
CSB-MP424256CWS
CSB-EP424256CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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