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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O9:H4 D-tagatose-1,6-bisphosphate aldolase subunit gatZ (gatZ) CSB-YP425180EJF
CSB-EP425180EJF
CSB-BP425180EJF
CSB-MP425180EJF
CSB-EP425180EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Periplasmic nitrate reductase (napA) CSB-YP425181EJF
CSB-EP425181EJF
CSB-BP425181EJF
CSB-MP425181EJF
CSB-EP425181EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 CinA-like protein (EcHS_A2392) CSB-YP425182EJF
CSB-EP425182EJF
CSB-BP425182EJF
CSB-MP425182EJF
CSB-EP425182EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP425183EJF1
CSB-EP425183EJF1
CSB-BP425183EJF1
CSB-MP425183EJF1
CSB-EP425183EJF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP425184EJF
CSB-EP425184EJF
CSB-BP425184EJF
CSB-MP425184EJF
CSB-EP425184EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP425185EJF
CSB-EP425185EJF
CSB-BP425185EJF
CSB-MP425185EJF
CSB-EP425185EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Putative ion-transport protein yfeO (yfeO), partial CSB-YP425186EJF
CSB-EP425186EJF
CSB-BP425186EJF
CSB-MP425186EJF
CSB-EP425186EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP425187EJF
CSB-EP425187EJF
CSB-BP425187EJF
CSB-MP425187EJF
CSB-EP425187EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 DnaA-homolog protein hda (hda) CSB-YP425188EJF
CSB-EP425188EJF
CSB-BP425188EJF
CSB-MP425188EJF
CSB-EP425188EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Histidine--tRNA ligase (hisS) CSB-YP425189EJF
CSB-EP425189EJF
CSB-BP425189EJF
CSB-MP425189EJF
CSB-EP425189EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP425190EJF
CSB-EP425190EJF
CSB-BP425190EJF
CSB-MP425190EJF
CSB-EP425190EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Protein grpE (grpE) CSB-YP425191EJF
CSB-EP425191EJF
CSB-BP425191EJF
CSB-MP425191EJF
CSB-EP425191EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Carbon storage regulator (csrA) CSB-YP425192EJF
CSB-EP425192EJF
CSB-BP425192EJF
CSB-MP425192EJF
CSB-EP425192EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Anaerobic nitric oxide reductase transcription regulator norR (norR) CSB-YP425193EJF
CSB-EP425193EJF
CSB-BP425193EJF
CSB-MP425193EJF
CSB-EP425193EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP425194EJF
CSB-EP425194EJF
CSB-BP425194EJF
CSB-MP425194EJF
CSB-EP425194EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 DNA mismatch repair protein mutH (mutH) CSB-YP425195EJF
CSB-EP425195EJF
CSB-BP425195EJF
CSB-MP425195EJF
CSB-EP425195EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 UPF0267 protein yqfB (yqfB) CSB-YP425196EJF
CSB-EP425196EJF
CSB-BP425196EJF
CSB-MP425196EJF
CSB-EP425196EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Phosphoglycerate kinase (pgk) CSB-YP425197EJF
CSB-EP425197EJF
CSB-BP425197EJF
CSB-MP425197EJF
CSB-EP425197EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Membrane-bound lytic murein transglycosylase C (mltC) CSB-YP425198EJF
CSB-EP425198EJF
CSB-BP425198EJF
CSB-MP425198EJF
CSB-EP425198EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Multifunctional CCA protein (cca) CSB-YP425199EJF
CSB-EP425199EJF
CSB-BP425199EJF
CSB-MP425199EJF
CSB-EP425199EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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