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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus gordonii UPF0246 protein SGO_1307 (SGO_1307) CSB-YP425339FMN
CSB-EP425339FMN
CSB-BP425339FMN
CSB-MP425339FMN
CSB-EP425339FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Shikimate kinase (aroK) CSB-YP425340FMN
CSB-EP425340FMN
CSB-BP425340FMN
CSB-MP425340FMN
CSB-EP425340FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 3-dehydroquinate dehydratase (aroD) CSB-YP425341FMN
CSB-EP425341FMN
CSB-BP425341FMN
CSB-MP425341FMN
CSB-EP425341FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP425342FMN
CSB-EP425342FMN
CSB-BP425342FMN
CSB-MP425342FMN
CSB-EP425342FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii D-alanine--D-alanine ligase (ddl) CSB-YP425343FMN
CSB-EP425343FMN
CSB-BP425343FMN
CSB-MP425343FMN
CSB-EP425343FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Alanine--tRNA ligase (alaS), partial CSB-YP425344FMN
CSB-EP425344FMN
CSB-BP425344FMN
CSB-MP425344FMN
CSB-EP425344FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Putative membrane protein insertion efficiency factor (SGO_1668) CSB-YP425345FMN
CSB-EP425345FMN
CSB-BP425345FMN
CSB-MP425345FMN
CSB-EP425345FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Glutamate racemase (murI) CSB-YP425346FMN
CSB-EP425346FMN
CSB-BP425346FMN
CSB-MP425346FMN
CSB-EP425346FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Imidazolonepropionase (hutI) CSB-YP425347FMN
CSB-EP425347FMN
CSB-BP425347FMN
CSB-MP425347FMN
CSB-EP425347FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii UPF0310 protein SGO_1818 (SGO_1818) CSB-YP425348FMN
CSB-EP425348FMN
CSB-BP425348FMN
CSB-MP425348FMN
CSB-EP425348FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP425349FMN
CSB-EP425349FMN
CSB-BP425349FMN
CSB-MP425349FMN
CSB-EP425349FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 50S ribosomal protein L15 (rplO) CSB-YP425350FMN
CSB-EP425350FMN
CSB-BP425350FMN
CSB-MP425350FMN
CSB-EP425350FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 30S ribosomal protein S17 (rpsQ) CSB-YP425351FMN
CSB-EP425351FMN
CSB-BP425351FMN
CSB-MP425351FMN
CSB-EP425351FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii 30S ribosomal protein S3 (rpsC) CSB-YP425352FMN
CSB-EP425352FMN
CSB-BP425352FMN
CSB-MP425352FMN
CSB-EP425352FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii UPF0473 protein SGO_2040 (SGO_2040) CSB-YP425353FMN
CSB-EP425353FMN
CSB-BP425353FMN
CSB-MP425353FMN
CSB-EP425353FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Histidine--tRNA ligase (hisS) CSB-YP425354FMN
CSB-EP425354FMN
CSB-BP425354FMN
CSB-MP425354FMN
CSB-EP425354FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis Structure-specific endonuclease subunit SLX1 homolog (GL50803_16475) CSB-YP425355GFY
CSB-EP425355GFY
CSB-BP425355GFY
CSB-MP425355GFY
CSB-EP425355GFY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 2 (M2), partial CSB-YP425356ILV1
CSB-EP425356ILV1
CSB-BP425356ILV1
CSB-MP425356ILV1
CSB-EP425356ILV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP425357ILP
CSB-EP425357ILP
CSB-BP425357ILP
CSB-MP425357ILP
CSB-EP425357ILP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial CSB-YP425358ILP
CSB-EP425358ILP
CSB-BP425358ILP
CSB-MP425358ILP
CSB-EP425358ILP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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