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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Serratia proteamaculans UPF0145 protein Spro_1658 (Spro_1658) CSB-YP424638STJ
CSB-EP424638STJ
CSB-BP424638STJ
CSB-MP424638STJ
CSB-EP424638STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Cytidylate kinase (cmk) CSB-YP424639STJ
CSB-EP424639STJ
CSB-BP424639STJ
CSB-MP424639STJ
CSB-EP424639STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Chromosome partition protein MukB (mukB), partial CSB-YP424640STJ
CSB-EP424640STJ
CSB-BP424640STJ
CSB-MP424640STJ
CSB-EP424640STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP424641STJ
CSB-EP424641STJ
CSB-BP424641STJ
CSB-MP424641STJ
CSB-EP424641STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Heat shock protein hspQ (hspQ) CSB-YP424642STJ
CSB-EP424642STJ
CSB-BP424642STJ
CSB-MP424642STJ
CSB-EP424642STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Putative aminoacrylate hydrolase RutD (rutD) CSB-YP424643STJ
CSB-EP424643STJ
CSB-BP424643STJ
CSB-MP424643STJ
CSB-EP424643STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Glucans biosynthesis protein G (mdoG) CSB-YP424644STJ
CSB-EP424644STJ
CSB-BP424644STJ
CSB-MP424644STJ
CSB-EP424644STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Probable phosphatase Spro_1934 (Spro_1934) CSB-YP424645STJ
CSB-EP424645STJ
CSB-BP424645STJ
CSB-MP424645STJ
CSB-EP424645STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Integration host factor subunit alpha (ihfA) CSB-YP424646STJ
CSB-EP424646STJ
CSB-BP424646STJ
CSB-MP424646STJ
CSB-EP424646STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP424647STJ
CSB-EP424647STJ
CSB-BP424647STJ
CSB-MP424647STJ
CSB-EP424647STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP424648STJ
CSB-EP424648STJ
CSB-BP424648STJ
CSB-MP424648STJ
CSB-EP424648STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans L-arabinose isomerase (araA), partial CSB-YP424649STJ
CSB-EP424649STJ
CSB-BP424649STJ
CSB-MP424649STJ
CSB-EP424649STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Flagellar brake protein YcgR (ycgR) CSB-YP424650STJ
CSB-EP424650STJ
CSB-BP424650STJ
CSB-MP424650STJ
CSB-EP424650STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Tryptophan synthase alpha chain (trpA) CSB-YP424651STJ
CSB-EP424651STJ
CSB-BP424651STJ
CSB-MP424651STJ
CSB-EP424651STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0263 protein Spro_2690 (Spro_2690) CSB-YP424652STJ
CSB-EP424652STJ
CSB-BP424652STJ
CSB-MP424652STJ
CSB-EP424652STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Na (+)/H (+) antiporter nhaB, partial CSB-YP424653STJ
CSB-EP424653STJ
CSB-BP424653STJ
CSB-MP424653STJ
CSB-EP424653STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Spermidine export protein MdtJ (mdtJ), partial CSB-YP424654STJ1
CSB-EP424654STJ1
CSB-BP424654STJ1
CSB-MP424654STJ1
CSB-EP424654STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans tRNA (mo5U34)-methyltransferase CSB-YP424655STJ
CSB-EP424655STJ
CSB-BP424655STJ
CSB-MP424655STJ
CSB-EP424655STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0266 membrane protein Spro_2816 (Spro_2816), partial CSB-YP424656STJ1
CSB-EP424656STJ1
CSB-BP424656STJ1
CSB-MP424656STJ1
CSB-EP424656STJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Flagellar transcriptional regulator FlhD (flhD) CSB-YP424657STJ
CSB-EP424657STJ
CSB-BP424657STJ
CSB-MP424657STJ
CSB-EP424657STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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