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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Azorhizobium caulinodans NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP424859DPR1
CSB-EP424859DPR1
CSB-BP424859DPR1
CSB-MP424859DPR1
CSB-EP424859DPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP424860DPR
CSB-EP424860DPR
CSB-BP424860DPR
CSB-MP424860DPR
CSB-EP424860DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans L-rhamnonate dehydratase (rhmD) CSB-YP424861DPR
CSB-EP424861DPR
CSB-BP424861DPR
CSB-MP424861DPR
CSB-EP424861DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans 60 kDa chaperonin 2 (groL2), partial CSB-YP424862DPR
CSB-EP424862DPR
CSB-BP424862DPR
CSB-MP424862DPR
CSB-EP424862DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Heme A synthase (ctaA), partial CSB-YP424863DPR
CSB-EP424863DPR
CSB-BP424863DPR
CSB-MP424863DPR
CSB-EP424863DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Anthranilate phosphoribosyltransferase (trpD) CSB-YP424864DPR
CSB-EP424864DPR
CSB-BP424864DPR
CSB-MP424864DPR
CSB-EP424864DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Deoxyguanosinetriphosphate triphosphohydrolase-like protein (AZC_2807) CSB-YP424865DPR
CSB-EP424865DPR
CSB-BP424865DPR
CSB-MP424865DPR
CSB-EP424865DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Putative aminoacrylate hydrolase RutD (rutD) CSB-YP424866DPR
CSB-EP424866DPR
CSB-BP424866DPR
CSB-MP424866DPR
CSB-EP424866DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans 30S ribosomal protein S8 (rpsH) CSB-YP424867DPR
CSB-EP424867DPR
CSB-BP424867DPR
CSB-MP424867DPR
CSB-EP424867DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans 30S ribosomal protein S3 (rpsC) CSB-YP424868DPR
CSB-EP424868DPR
CSB-BP424868DPR
CSB-MP424868DPR
CSB-EP424868DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans UPF0262 protein AZC_3148 (AZC_3148) CSB-YP424869DPR
CSB-EP424869DPR
CSB-BP424869DPR
CSB-MP424869DPR
CSB-EP424869DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP424870DPR
CSB-EP424870DPR
CSB-BP424870DPR
CSB-MP424870DPR
CSB-EP424870DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans C4-dicarboxylate transport protein (dctA), partial CSB-YP424871DPR
CSB-EP424871DPR
CSB-BP424871DPR
CSB-MP424871DPR
CSB-EP424871DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP424872DPR
CSB-EP424872DPR
CSB-BP424872DPR
CSB-MP424872DPR
CSB-EP424872DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Thiamine-phosphate synthase (thiE) CSB-YP424873DPR
CSB-EP424873DPR
CSB-BP424873DPR
CSB-MP424873DPR
CSB-EP424873DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Recombination protein RecR (recR) CSB-YP424874DPR
CSB-EP424874DPR
CSB-BP424874DPR
CSB-MP424874DPR
CSB-EP424874DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans Catalase-peroxidase (katG), partial CSB-YP424875DPR
CSB-EP424875DPR
CSB-BP424875DPR
CSB-MP424875DPR
CSB-EP424875DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans 50S ribosomal protein L32 (rpmF) CSB-YP424876DPR
CSB-EP424876DPR
CSB-BP424876DPR
CSB-MP424876DPR
CSB-EP424876DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans 50S ribosomal protein L19 (rplS) CSB-YP424877DPR
CSB-EP424877DPR
CSB-BP424877DPR
CSB-MP424877DPR
CSB-EP424877DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azorhizobium caulinodans 50S ribosomal protein L34 (rpmH) CSB-YP424878DPR
CSB-EP424878DPR
CSB-BP424878DPR
CSB-MP424878DPR
CSB-EP424878DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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