Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus amyloliquefaciens 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP424999BQD
CSB-EP424999BQD
CSB-BP424999BQD
CSB-MP424999BQD
CSB-EP424999BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Tryptophan synthase alpha chain (trpA) CSB-YP425000BQD
CSB-EP425000BQD
CSB-BP425000BQD
CSB-MP425000BQD
CSB-EP425000BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP425001BQD
CSB-EP425001BQD
CSB-BP425001BQD
CSB-MP425001BQD
CSB-EP425001BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP425002BQD
CSB-EP425002BQD
CSB-BP425002BQD
CSB-MP425002BQD
CSB-EP425002BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP425003BQD
CSB-EP425003BQD
CSB-BP425003BQD
CSB-MP425003BQD
CSB-EP425003BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP425004BQD
CSB-EP425004BQD
CSB-BP425004BQD
CSB-MP425004BQD
CSB-EP425004BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Elongation factor 4 (lepA), partial CSB-YP425005BQD
CSB-EP425005BQD
CSB-BP425005BQD
CSB-MP425005BQD
CSB-EP425005BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UPF0473 protein RBAM_024480 (RBAM_024480) CSB-YP425006BQD
CSB-EP425006BQD
CSB-BP425006BQD
CSB-MP425006BQD
CSB-EP425006BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Aspartate--tRNA ligase (aspS), partial CSB-YP425007BQD
CSB-EP425007BQD
CSB-BP425007BQD
CSB-MP425007BQD
CSB-EP425007BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UPF0735 ACT domain-containing protein RBAM_024960 (RBAM_024960) CSB-YP425008BQD
CSB-EP425008BQD
CSB-BP425008BQD
CSB-MP425008BQD
CSB-EP425008BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Glutamate-1-semialdehyde 2,1-aminomutase 2 (hemL2) CSB-YP425009BQD
CSB-EP425009BQD
CSB-BP425009BQD
CSB-MP425009BQD
CSB-EP425009BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 2-isopropylmalate synthase (leuA), partial CSB-YP425010BQD
CSB-EP425010BQD
CSB-BP425010BQD
CSB-MP425010BQD
CSB-EP425010BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP425011BQD
CSB-EP425011BQD
CSB-BP425011BQD
CSB-MP425011BQD
CSB-EP425011BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 30S ribosomal protein S4 (rpsD) CSB-YP425012BQD
CSB-EP425012BQD
CSB-BP425012BQD
CSB-MP425012BQD
CSB-EP425012BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP425013BQD
CSB-EP425013BQD
CSB-BP425013BQD
CSB-MP425013BQD
CSB-EP425013BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Probable cytosol aminopeptidase (pepA) CSB-YP425014BQD
CSB-EP425014BQD
CSB-BP425014BQD
CSB-MP425014BQD
CSB-EP425014BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP425015BQD
CSB-EP425015BQD
CSB-BP425015BQD
CSB-MP425015BQD
CSB-EP425015BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens UvrABC system protein B (uvrB), partial CSB-YP425016BQD
CSB-EP425016BQD
CSB-BP425016BQD
CSB-MP425016BQD
CSB-EP425016BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP425017BQD
CSB-EP425017BQD
CSB-BP425017BQD
CSB-MP425017BQD
CSB-EP425017BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Octanoyl-[GcvH]:protein N-octanoyltransferase (lipL) CSB-YP425018BQD
CSB-EP425018BQD
CSB-BP425018BQD
CSB-MP425018BQD
CSB-EP425018BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>