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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella sediminis Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP426579STR
CSB-EP426579STR
CSB-BP426579STR
CSB-MP426579STR
CSB-EP426579STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Cytochrome c-552 (nrfA) CSB-YP426580STR
CSB-EP426580STR
CSB-BP426580STR
CSB-MP426580STR
CSB-EP426580STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Protein ApaG (apaG) CSB-YP426581STR
CSB-EP426581STR
CSB-BP426581STR
CSB-MP426581STR
CSB-EP426581STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP426582STR
CSB-EP426582STR
CSB-BP426582STR
CSB-MP426582STR
CSB-EP426582STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 5'-nucleotidase surE (surE) CSB-YP426583STR
CSB-EP426583STR
CSB-BP426583STR
CSB-MP426583STR
CSB-EP426583STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis UPF0178 protein Ssed_1350 (Ssed_1350) CSB-YP426584STR
CSB-EP426584STR
CSB-BP426584STR
CSB-MP426584STR
CSB-EP426584STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis GTPase Der (der) CSB-YP426585STR
CSB-EP426585STR
CSB-BP426585STR
CSB-MP426585STR
CSB-EP426585STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP426586STR
CSB-EP426586STR
CSB-BP426586STR
CSB-MP426586STR
CSB-EP426586STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP426587STR
CSB-EP426587STR
CSB-BP426587STR
CSB-MP426587STR
CSB-EP426587STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Protein TolB (tolB) CSB-YP426588STR
CSB-EP426588STR
CSB-BP426588STR
CSB-MP426588STR
CSB-EP426588STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP426589STR
CSB-EP426589STR
CSB-BP426589STR
CSB-MP426589STR
CSB-EP426589STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis tRNA (mo5U34)-methyltransferase CSB-YP426590STR
CSB-EP426590STR
CSB-BP426590STR
CSB-MP426590STR
CSB-EP426590STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Electron transport complex protein RnfD (rnfD), partial CSB-YP426591STR
CSB-EP426591STR
CSB-BP426591STR
CSB-MP426591STR
CSB-EP426591STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis 50S ribosomal protein L20 (rplT) CSB-YP426592STR
CSB-EP426592STR
CSB-BP426592STR
CSB-MP426592STR
CSB-EP426592STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP426593STR
CSB-EP426593STR
CSB-BP426593STR
CSB-MP426593STR
CSB-EP426593STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Asparagine--tRNA ligase (asnS) CSB-YP426594STR
CSB-EP426594STR
CSB-BP426594STR
CSB-MP426594STR
CSB-EP426594STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Na (+)/H (+) antiporter nhaB, partial CSB-YP426595STR
CSB-EP426595STR
CSB-BP426595STR
CSB-MP426595STR
CSB-EP426595STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis ATP phosphoribosyltransferase (hisG) CSB-YP426596STR
CSB-EP426596STR
CSB-BP426596STR
CSB-MP426596STR
CSB-EP426596STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Phosphate acyltransferase (plsX) CSB-YP426597STR
CSB-EP426597STR
CSB-BP426597STR
CSB-MP426597STR
CSB-EP426597STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Protein-L-isoaspartate O-methyltransferase 2 (pcm2) CSB-YP426598STR
CSB-EP426598STR
CSB-BP426598STR
CSB-MP426598STR
CSB-EP426598STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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