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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sulcia muelleri 50S ribosomal protein L22 (rplV) CSB-YP428380SUX
CSB-EP428380SUX
CSB-BP428380SUX
CSB-MP428380SUX
CSB-EP428380SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri 50S ribosomal protein L16 (rplP) CSB-YP428381SUX
CSB-EP428381SUX
CSB-BP428381SUX
CSB-MP428381SUX
CSB-EP428381SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri 30S ribosomal protein S8 (rpsH) CSB-YP428382SUX
CSB-EP428382SUX
CSB-BP428382SUX
CSB-MP428382SUX
CSB-EP428382SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri 30S ribosomal protein S13 (rpsM) CSB-YP428383SUX
CSB-EP428383SUX
CSB-BP428383SUX
CSB-MP428383SUX
CSB-EP428383SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulcia muelleri Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP428384SUX
CSB-EP428384SUX
CSB-BP428384SUX
CSB-MP428384SUX
CSB-EP428384SUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP428385CWA
CSB-EP428385CWA
CSB-BP428385CWA
CSB-MP428385CWA
CSB-EP428385CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus 30S ribosomal protein S12 (rpsL) CSB-YP428386CWA
CSB-EP428386CWA
CSB-BP428386CWA
CSB-MP428386CWA
CSB-EP428386CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Indole-3-glycerol phosphate synthase (trpC) CSB-YP428387CWA
CSB-EP428387CWA
CSB-BP428387CWA
CSB-MP428387CWA
CSB-EP428387CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP428388CWA
CSB-EP428388CWA
CSB-BP428388CWA
CSB-MP428388CWA
CSB-EP428388CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 30S ribosomal protein S18 (rpsR) CSB-YP428389DHW
CSB-EP428389DHW
CSB-BP428389DHW
CSB-MP428389DHW
CSB-EP428389DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 50S ribosomal protein L34 (rpmH) CSB-YP428390DHW
CSB-EP428390DHW
CSB-BP428390DHW
CSB-MP428390DHW
CSB-EP428390DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP428391DHW
CSB-EP428391DHW
CSB-BP428391DHW
CSB-MP428391DHW
CSB-EP428391DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP428392DHW
CSB-EP428392DHW
CSB-BP428392DHW
CSB-MP428392DHW
CSB-EP428392DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Adenylosuccinate synthetase (purA) CSB-YP428393DHW
CSB-EP428393DHW
CSB-BP428393DHW
CSB-MP428393DHW
CSB-EP428393DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans UPF0042 nucleotide-binding protein Dole_0503 (Dole_0503) CSB-YP428394DHW
CSB-EP428394DHW
CSB-BP428394DHW
CSB-MP428394DHW
CSB-EP428394DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 50S ribosomal protein L28 (rpmB) CSB-YP428395DHW
CSB-EP428395DHW
CSB-BP428395DHW
CSB-MP428395DHW
CSB-EP428395DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 10 kDa chaperonin (groS) CSB-YP428396DHW
CSB-EP428396DHW
CSB-BP428396DHW
CSB-MP428396DHW
CSB-EP428396DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans ATP synthase epsilon chain (atpC) CSB-YP428397DHW
CSB-EP428397DHW
CSB-BP428397DHW
CSB-MP428397DHW
CSB-EP428397DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP428398DHW
CSB-EP428398DHW
CSB-BP428398DHW
CSB-MP428398DHW
CSB-EP428398DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans D-tyrosyl-tRNA (Tyr) deacylase CSB-YP428399DHW
CSB-EP428399DHW
CSB-BP428399DHW
CSB-MP428399DHW
CSB-EP428399DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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