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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio Aminoglycoside phosphotransferase domain-containing protein 1 (agphd1) CSB-YP428180DIL
CSB-BP428180DIL
CSB-MP428180DIL
CSB-EP428180DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Leucine-rich repeat and fibronectin type III domain-containing protein 1-like protein (lrfn1l), partial CSB-YP428181DIL
CSB-EP428181DIL
CSB-BP428181DIL
CSB-MP428181DIL
CSB-EP428181DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kinetochore null protein 1 (knl-1), partial CSB-YP428182CXX
CSB-EP428182CXX
CSB-BP428182CXX
CSB-MP428182CXX
CSB-EP428182CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant E3 UFM1-protein ligase 1 homolog (CBG00415), partial CSB-YP428183CXX
CSB-EP428183CXX
CSB-BP428183CXX
CSB-MP428183CXX
CSB-EP428183CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetylcholine receptor subunit alpha-type acr-16 (acr-16), partial CSB-YP428184CXX1
CSB-EP428184CXX1
CSB-BP428184CXX1
CSB-MP428184CXX1
CSB-EP428184CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable DNA-directed RNA polymerases I, II, and III subunit RPABC2 (rpb-6) CSB-YP428185CXX
CSB-EP428185CXX
CSB-BP428185CXX
CSB-MP428185CXX
CSB-EP428185CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histone-lysine N-methyltransferase Suv4-20 (set-4) CSB-YP428186CXX
CSB-EP428186CXX
CSB-BP428186CXX
CSB-MP428186CXX
CSB-EP428186CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable dolichol-phosphate mannosyltransferase subunit 3 (dpm-3), partial CSB-YP428187CXX1
CSB-EP428187CXX1
CSB-BP428187CXX1
CSB-MP428187CXX1
CSB-EP428187CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Receptor-mediated endocytosis protein 6 (rme-6), partial CSB-YP428188CXX
CSB-EP428188CXX
CSB-BP428188CXX
CSB-MP428188CXX
CSB-EP428188CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mitogen-activated protein kinase kinase kinase mom-4 (mom-4), partial CSB-YP428189CXX
CSB-EP428189CXX
CSB-BP428189CXX
CSB-MP428189CXX
CSB-EP428189CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Progestin and adipoq receptor-like protein 1 (paqr-1), partial CSB-YP428190CXX1
CSB-EP428190CXX1
CSB-BP428190CXX1
CSB-MP428190CXX1
CSB-EP428190CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable queuine tRNA-ribosyltransferase (tgt-1) CSB-YP428191CXX
CSB-EP428191CXX
CSB-BP428191CXX
CSB-MP428191CXX
CSB-EP428191CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zinc finger protein-like 1 homolog (CBG06644), partial CSB-YP428192CXX1
CSB-EP428192CXX1
CSB-BP428192CXX1
CSB-MP428192CXX1
CSB-EP428192CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant APC-related protein 1 (apr-1), partial CSB-YP428193CXX
CSB-EP428193CXX
CSB-BP428193CXX
CSB-MP428193CXX
CSB-EP428193CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cytochrome c 2.2 (cyc-2.2) CSB-YP428194CXX
CSB-EP428194CXX
CSB-BP428194CXX
CSB-MP428194CXX
CSB-EP428194CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidyl-prolyl cis-trans isomerase 9 (cyn-9) CSB-YP428195CXX
CSB-EP428195CXX
CSB-BP428195CXX
CSB-MP428195CXX
CSB-EP428195CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barrier-to-autointegration factor 1 (baf-1) CSB-YP428196CXX
CSB-EP428196CXX
CSB-BP428196CXX
CSB-MP428196CXX
CSB-EP428196CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable small nuclear ribonucleoprotein E (snr-6) CSB-YP428197CXX
CSB-EP428197CXX
CSB-BP428197CXX
CSB-MP428197CXX
CSB-EP428197CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell death abnormality protein 12 (ced-12), partial CSB-YP428198CXX
CSB-EP428198CXX
CSB-BP428198CXX
CSB-MP428198CXX
CSB-EP428198CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Axin-like protein 1 (axl-1) CSB-YP428199CXX
CSB-EP428199CXX
CSB-BP428199CXX
CSB-MP428199CXX
CSB-EP428199CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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