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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella canis Urease accessory protein UreG 2 (ureG2) CSB-YP429400BPH
CSB-EP429400BPH
CSB-BP429400BPH
CSB-MP429400BPH
CSB-EP429400BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Ribosomal protein L11 methyltransferase (prmA) CSB-YP429401BPH
CSB-EP429401BPH
CSB-BP429401BPH
CSB-MP429401BPH
CSB-EP429401BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Trans-aconitate 2-methyltransferase (tam) CSB-YP429402BPH
CSB-EP429402BPH
CSB-BP429402BPH
CSB-MP429402BPH
CSB-EP429402BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP429403BPH
CSB-EP429403BPH
CSB-BP429403BPH
CSB-MP429403BPH
CSB-EP429403BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP429404BPH
CSB-EP429404BPH
CSB-BP429404BPH
CSB-MP429404BPH
CSB-EP429404BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP429405BPH
CSB-EP429405BPH
CSB-BP429405BPH
CSB-MP429405BPH
CSB-EP429405BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Elongation factor P (efp) CSB-YP429406BPH
CSB-EP429406BPH
CSB-BP429406BPH
CSB-MP429406BPH
CSB-EP429406BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP429407BPH
CSB-EP429407BPH
CSB-BP429407BPH
CSB-MP429407BPH
CSB-EP429407BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Protein ApaG (apaG) CSB-YP429408BPH
CSB-EP429408BPH
CSB-BP429408BPH
CSB-MP429408BPH
CSB-EP429408BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Probable transaldolase (tal) CSB-YP429409BPH
CSB-EP429409BPH
CSB-BP429409BPH
CSB-MP429409BPH
CSB-EP429409BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 30S ribosomal protein S16 (rpsP) CSB-YP429410BPH
CSB-EP429410BPH
CSB-BP429410BPH
CSB-MP429410BPH
CSB-EP429410BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis GTPase obg (obg) CSB-YP429411BPH
CSB-EP429411BPH
CSB-BP429411BPH
CSB-MP429411BPH
CSB-EP429411BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Large-conductance mechanosensitive channel (mscL), partial CSB-YP429412BPH1
CSB-EP429412BPH1
CSB-BP429412BPH1
CSB-MP429412BPH1
CSB-EP429412BPH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis ATP synthase subunit b 1 (atpF1), partial CSB-YP429413BPH1
CSB-EP429413BPH1
CSB-BP429413BPH1
CSB-MP429413BPH1
CSB-EP429413BPH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP429414BPH
CSB-EP429414BPH
CSB-BP429414BPH
CSB-MP429414BPH
CSB-EP429414BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Xylose isomerase (xylA) CSB-YP429415BPH
CSB-EP429415BPH
CSB-BP429415BPH
CSB-MP429415BPH
CSB-EP429415BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP429416BPH
CSB-EP429416BPH
CSB-BP429416BPH
CSB-MP429416BPH
CSB-EP429416BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP429417BPH
CSB-EP429417BPH
CSB-BP429417BPH
CSB-MP429417BPH
CSB-EP429417BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 50S ribosomal protein L35 (rpmI) CSB-YP429418BPH
CSB-EP429418BPH
CSB-BP429418BPH
CSB-MP429418BPH
CSB-EP429418BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP429419BPH
CSB-EP429419BPH
CSB-BP429419BPH
CSB-MP429419BPH
CSB-EP429419BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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