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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia multivorans UPF0434 protein Bmul_0750/BMULJ_02510 (Bmul_0750, BMULJ_02510) CSB-YP429000BPS
CSB-EP429000BPS
CSB-BP429000BPS
CSB-MP429000BPS
CSB-EP429000BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Probable Fe (2+)-trafficking protein CSB-YP429001BPS
CSB-EP429001BPS
CSB-BP429001BPS
CSB-MP429001BPS
CSB-EP429001BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UPF0133 protein Bmul_1447/BMULJ_01796 (Bmul_1447, BMULJ_01796) CSB-YP429002BPS
CSB-EP429002BPS
CSB-BP429002BPS
CSB-MP429002BPS
CSB-EP429002BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP429003BPS
CSB-EP429003BPS
CSB-BP429003BPS
CSB-MP429003BPS
CSB-EP429003BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Leucine--tRNA ligase (leuS), partial CSB-YP429004BPS
CSB-EP429004BPS
CSB-BP429004BPS
CSB-MP429004BPS
CSB-EP429004BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Methionine--tRNA ligase (metG), partial CSB-YP429005BPS
CSB-EP429005BPS
CSB-BP429005BPS
CSB-MP429005BPS
CSB-EP429005BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP429006BPS
CSB-EP429006BPS
CSB-BP429006BPS
CSB-MP429006BPS
CSB-EP429006BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L21 (rplU) CSB-YP429007BPS
CSB-EP429007BPS
CSB-BP429007BPS
CSB-MP429007BPS
CSB-EP429007BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP429008BPS
CSB-EP429008BPS
CSB-BP429008BPS
CSB-MP429008BPS
CSB-EP429008BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UPF0303 protein Bmul_1674/BMULJ_01570 (Bmul_1674, BMULJ_01570) CSB-YP429009BPS
CSB-EP429009BPS
CSB-BP429009BPS
CSB-MP429009BPS
CSB-EP429009BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Glutamyl-tRNA reductase (hemA) CSB-YP429010BPS
CSB-EP429010BPS
CSB-BP429010BPS
CSB-MP429010BPS
CSB-EP429010BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Uracil phosphoribosyltransferase (upp) CSB-YP429011BPS
CSB-EP429011BPS
CSB-BP429011BPS
CSB-MP429011BPS
CSB-EP429011BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP429012BPS1
CSB-EP429012BPS1
CSB-BP429012BPS1
CSB-MP429012BPS1
CSB-EP429012BPS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L9 (rplI) CSB-YP429013BPS
CSB-EP429013BPS
CSB-BP429013BPS
CSB-MP429013BPS
CSB-EP429013BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP429014BPS
CSB-EP429014BPS
CSB-BP429014BPS
CSB-MP429014BPS
CSB-EP429014BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans GTP cyclohydrolase folE2 (folE2) CSB-YP429015BPS
CSB-EP429015BPS
CSB-BP429015BPS
CSB-MP429015BPS
CSB-EP429015BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Aspartate 1-decarboxylase (panD) CSB-YP429016HSK
CSB-EP429016HSK
CSB-BP429016HSK
CSB-MP429016HSK
CSB-EP429016HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus ATP synthase gamma chain (atpG) CSB-YP429017HSK
CSB-EP429017HSK
CSB-BP429017HSK
CSB-MP429017HSK
CSB-EP429017HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Chorismate synthase (aroC) CSB-YP429018HSK
CSB-EP429018HSK
CSB-BP429018HSK
CSB-MP429018HSK
CSB-EP429018HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Glutamate--tRNA ligase (gltX) CSB-YP429019HSK
CSB-EP429019HSK
CSB-BP429019HSK
CSB-MP429019HSK
CSB-EP429019HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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