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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Desulfococcus oleovorans ATP synthase subunit c (atpE), partial CSB-YP428400DHW1
CSB-EP428400DHW1
CSB-BP428400DHW1
CSB-MP428400DHW1
CSB-EP428400DHW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Histidine--tRNA ligase (hisS) CSB-YP428401DHW
CSB-EP428401DHW
CSB-BP428401DHW
CSB-MP428401DHW
CSB-EP428401DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP428402DHW
CSB-EP428402DHW
CSB-BP428402DHW
CSB-MP428402DHW
CSB-EP428402DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 50S ribosomal protein L1 (rplA) CSB-YP428403DHW
CSB-EP428403DHW
CSB-BP428403DHW
CSB-MP428403DHW
CSB-EP428403DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP428404DHW
CSB-EP428404DHW
CSB-BP428404DHW
CSB-MP428404DHW
CSB-EP428404DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 50S ribosomal protein L4 (rplD) CSB-YP428405DHW
CSB-EP428405DHW
CSB-BP428405DHW
CSB-MP428405DHW
CSB-EP428405DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 50S ribosomal protein L22 (rplV) CSB-YP428406DHW
CSB-EP428406DHW
CSB-BP428406DHW
CSB-MP428406DHW
CSB-EP428406DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 30S ribosomal protein S14 type Z (rpsZ) CSB-YP428407DHW
CSB-EP428407DHW
CSB-BP428407DHW
CSB-MP428407DHW
CSB-EP428407DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 50S ribosomal protein L18 (rplR) CSB-YP428408DHW
CSB-EP428408DHW
CSB-BP428408DHW
CSB-MP428408DHW
CSB-EP428408DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 30S ribosomal protein S4 (rpsD) CSB-YP428409DHW
CSB-EP428409DHW
CSB-BP428409DHW
CSB-MP428409DHW
CSB-EP428409DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP428410DHW
CSB-EP428410DHW
CSB-BP428410DHW
CSB-MP428410DHW
CSB-EP428410DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 30S ribosomal protein S20 (rpsT) CSB-YP428411DHW
CSB-EP428411DHW
CSB-BP428411DHW
CSB-MP428411DHW
CSB-EP428411DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP428412DHW
CSB-EP428412DHW
CSB-BP428412DHW
CSB-MP428412DHW
CSB-EP428412DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Probable GTP-binding protein EngB (engB) CSB-YP428413DHW
CSB-EP428413DHW
CSB-BP428413DHW
CSB-MP428413DHW
CSB-EP428413DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 5'-nucleotidase surE (surE) CSB-YP428414DHW
CSB-EP428414DHW
CSB-BP428414DHW
CSB-MP428414DHW
CSB-EP428414DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP428415DHW
CSB-EP428415DHW
CSB-BP428415DHW
CSB-MP428415DHW
CSB-EP428415DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP428416DHW
CSB-EP428416DHW
CSB-BP428416DHW
CSB-MP428416DHW
CSB-EP428416DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 50S ribosomal protein L25 (rplY) CSB-YP428417DHW
CSB-EP428417DHW
CSB-BP428417DHW
CSB-MP428417DHW
CSB-EP428417DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP428418DHW
CSB-EP428418DHW
CSB-BP428418DHW
CSB-MP428418DHW
CSB-EP428418DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfococcus oleovorans 30S ribosomal protein S15 (rpsO) CSB-YP428419DHW
CSB-EP428419DHW
CSB-BP428419DHW
CSB-MP428419DHW
CSB-EP428419DHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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