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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella arizonae Elongation factor P (efp) CSB-YP429440STE
CSB-EP429440STE
CSB-BP429440STE
CSB-MP429440STE
CSB-EP429440STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Anaerobic nitric oxide reductase flavorubredoxin (norV) CSB-YP429441STE
CSB-EP429441STE
CSB-BP429441STE
CSB-MP429441STE
CSB-EP429441STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae L-alanine exporter AlaE (alaE), partial CSB-YP429442STE1
CSB-EP429442STE1
CSB-BP429442STE1
CSB-MP429442STE1
CSB-EP429442STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0227 protein ycfP (ycfP) CSB-YP429443STE
CSB-EP429443STE
CSB-BP429443STE
CSB-MP429443STE
CSB-EP429443STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribose 1,5-bisphosphate phosphokinase PhnN (phnN) CSB-YP429444STE
CSB-EP429444STE
CSB-BP429444STE
CSB-MP429444STE
CSB-EP429444STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP429445STE
CSB-EP429445STE
CSB-BP429445STE
CSB-MP429445STE
CSB-EP429445STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Dihydroorotase (pyrC) CSB-YP429446STE
CSB-EP429446STE
CSB-BP429446STE
CSB-MP429446STE
CSB-EP429446STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH) CSB-YP429447STE
CSB-EP429447STE
CSB-BP429447STE
CSB-MP429447STE
CSB-EP429447STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Endonuclease V (nfi) CSB-YP429448STE
CSB-EP429448STE
CSB-BP429448STE
CSB-MP429448STE
CSB-EP429448STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Thiamine-phosphate synthase (thiE) CSB-YP429449STE
CSB-EP429449STE
CSB-BP429449STE
CSB-MP429449STE
CSB-EP429449STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae HTH-type transcriptional regulator iscR (iscR) CSB-YP429450STE
CSB-EP429450STE
CSB-BP429450STE
CSB-MP429450STE
CSB-EP429450STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Chaperone protein hscA (hscA), partial CSB-YP429451STE
CSB-EP429451STE
CSB-BP429451STE
CSB-MP429451STE
CSB-EP429451STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0370 protein ypfN (ypfN), partial CSB-YP429452STE
CSB-EP429452STE
CSB-BP429452STE
CSB-MP429452STE
CSB-EP429452STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Methylglyoxal synthase (mgsA) CSB-YP429453STE
CSB-EP429453STE
CSB-BP429453STE
CSB-MP429453STE
CSB-EP429453STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Chromosome partition protein MukB (mukB), partial CSB-YP429454STE
CSB-EP429454STE
CSB-BP429454STE
CSB-MP429454STE
CSB-EP429454STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP429455STE
CSB-EP429455STE
CSB-BP429455STE
CSB-MP429455STE
CSB-EP429455STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Acetylornithine deacetylase (argE) CSB-YP429456STE
CSB-EP429456STE
CSB-BP429456STE
CSB-MP429456STE
CSB-EP429456STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae HTH-type transcriptional activator rhaR (rhaR) CSB-YP429457STE
CSB-EP429457STE
CSB-BP429457STE
CSB-MP429457STE
CSB-EP429457STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae DNA ligase (ligA), partial CSB-YP429458STE
CSB-EP429458STE
CSB-BP429458STE
CSB-MP429458STE
CSB-EP429458STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP429459STE
CSB-EP429459STE
CSB-BP429459STE
CSB-MP429459STE
CSB-EP429459STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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