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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus Anthranilate phosphoribosyltransferase (trpD) CSB-YP429040PZC
CSB-EP429040PZC
CSB-BP429040PZC
CSB-MP429040PZC
CSB-EP429040PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Histidine--tRNA ligase (hisS) CSB-YP429041PZC
CSB-EP429041PZC
CSB-BP429041PZC
CSB-MP429041PZC
CSB-EP429041PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP429042PZC
CSB-EP429042PZC
CSB-BP429042PZC
CSB-MP429042PZC
CSB-EP429042PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Putative Holliday junction resolvase (P9211_09701) CSB-YP429043PZC
CSB-EP429043PZC
CSB-BP429043PZC
CSB-MP429043PZC
CSB-EP429043PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Bifunctional protein GlmU (glmU) CSB-YP429044PZC
CSB-EP429044PZC
CSB-BP429044PZC
CSB-MP429044PZC
CSB-EP429044PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Bifunctional protein FolD (folD) CSB-YP429045PZC
CSB-EP429045PZC
CSB-BP429045PZC
CSB-MP429045PZC
CSB-EP429045PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S16 (rpsP) CSB-YP429046PZC
CSB-EP429046PZC
CSB-BP429046PZC
CSB-MP429046PZC
CSB-EP429046PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP429047PZC
CSB-EP429047PZC
CSB-BP429047PZC
CSB-MP429047PZC
CSB-EP429047PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus LL-diaminopimelate aminotransferase (dapL) CSB-YP429048PZC
CSB-EP429048PZC
CSB-BP429048PZC
CSB-MP429048PZC
CSB-EP429048PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L31 (rpmE) CSB-YP429049PZC
CSB-EP429049PZC
CSB-BP429049PZC
CSB-MP429049PZC
CSB-EP429049PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L36 (rpmJ) CSB-YP429050PZC
CSB-EP429050PZC
CSB-BP429050PZC
CSB-MP429050PZC
CSB-EP429050PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L29 (rpmC) CSB-YP429051PZC
CSB-EP429051PZC
CSB-BP429051PZC
CSB-MP429051PZC
CSB-EP429051PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L4 (rplD) CSB-YP429052PZC
CSB-EP429052PZC
CSB-BP429052PZC
CSB-MP429052PZC
CSB-EP429052PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phycoerythrobilin:ferredoxin oxidoreductase (pebB) CSB-YP429053PZC
CSB-EP429053PZC
CSB-BP429053PZC
CSB-MP429053PZC
CSB-EP429053PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I assembly protein ycf3 (ycf3) CSB-YP429054PZC
CSB-EP429054PZC
CSB-BP429054PZC
CSB-MP429054PZC
CSB-EP429054PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable rRNA maturation factor (P9211_02001) CSB-YP429055PZC
CSB-EP429055PZC
CSB-BP429055PZC
CSB-MP429055PZC
CSB-EP429055PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus SsrA-binding protein (smpB) CSB-YP429056PZC
CSB-EP429056PZC
CSB-BP429056PZC
CSB-MP429056PZC
CSB-EP429056PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Protein translocase subunit SecA (secA), partial CSB-YP429057PZC
CSB-EP429057PZC
CSB-BP429057PZC
CSB-MP429057PZC
CSB-EP429057PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphoglycerate kinase (pgk) CSB-YP429058PZC
CSB-EP429058PZC
CSB-BP429058PZC
CSB-MP429058PZC
CSB-EP429058PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Isoleucine--tRNA ligase (ileS), partial CSB-YP429059PZC
CSB-EP429059PZC
CSB-BP429059PZC
CSB-MP429059PZC
CSB-EP429059PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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