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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acholeplasma laidlawii 30S ribosomal protein S4 (rpsD) CSB-YP429640ATX
CSB-EP429640ATX
CSB-BP429640ATX
CSB-MP429640ATX
CSB-EP429640ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii 60 kDa chaperonin (groL), partial CSB-YP429641ATX
CSB-EP429641ATX
CSB-BP429641ATX
CSB-MP429641ATX
CSB-EP429641ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea sitchensis Costars family protein WS02710_H03 CSB-YP429642EVO
CSB-EP429642EVO
CSB-BP429642EVO
CSB-MP429642EVO
CSB-EP429642EVO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea sitchensis CASP-like protein 2, partial CSB-YP429643EVO1
CSB-EP429643EVO1
CSB-BP429643EVO1
CSB-MP429643EVO1
CSB-EP429643EVO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa Anamorsin homolog (POPTRDRAFT_761104) CSB-YP429644EXS
CSB-EP429644EXS
CSB-BP429644EXS
CSB-MP429644EXS
CSB-EP429644EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes DNA topoisomerase I, mitochondrial (TOP1MT), partial CSB-YP429645EQV
CSB-EP429645EQV
CSB-BP429645EQV
CSB-MP429645EQV
CSB-EP429645EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachelium caeruleum ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP429646TOW1
CSB-EP429646TOW1
CSB-BP429646TOW1
CSB-MP429646TOW1
CSB-EP429646TOW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachelium caeruleum NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic, partial CSB-YP429647TOW1
CSB-EP429647TOW1
CSB-BP429647TOW1
CSB-MP429647TOW1
CSB-EP429647TOW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachelium caeruleum Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP429648TOW1
CSB-EP429648TOW1
CSB-BP429648TOW1
CSB-MP429648TOW1
CSB-EP429648TOW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Low affinity potassium transport system protein kup (kup), partial CSB-YP429649YAM
CSB-EP429649YAM
CSB-BP429649YAM
CSB-MP429649YAM
CSB-EP429649YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Chorismate--pyruvate lyase (ubiC) CSB-YP429650YAM
CSB-EP429650YAM
CSB-BP429650YAM
CSB-MP429650YAM
CSB-EP429650YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Rhamnulokinase (rhaB) CSB-YP429651YAM
CSB-EP429651YAM
CSB-BP429651YAM
CSB-MP429651YAM
CSB-EP429651YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP429652YAM
CSB-EP429652YAM
CSB-BP429652YAM
CSB-MP429652YAM
CSB-EP429652YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP429653YAM
CSB-EP429653YAM
CSB-BP429653YAM
CSB-MP429653YAM
CSB-EP429653YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Outer-membrane lipoprotein lolB (lolB) CSB-YP429654YAM
CSB-EP429654YAM
CSB-BP429654YAM
CSB-MP429654YAM
CSB-EP429654YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Putative sialic acid transporter (nanT), partial CSB-YP429655YAM
CSB-EP429655YAM
CSB-BP429655YAM
CSB-MP429655YAM
CSB-EP429655YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0299 membrane protein YpAngola_A3025 (YpAngola_A3025), partial CSB-YP429656YAM1
CSB-EP429656YAM1
CSB-BP429656YAM1
CSB-MP429656YAM1
CSB-EP429656YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP429657YAM
CSB-EP429657YAM
CSB-BP429657YAM
CSB-MP429657YAM
CSB-EP429657YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Protein ApaG (apaG) CSB-YP429658YAM
CSB-EP429658YAM
CSB-BP429658YAM
CSB-MP429658YAM
CSB-EP429658YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Na (+)/H (+) antiporter nhaA, partial CSB-YP429659YAM
CSB-EP429659YAM
CSB-BP429659YAM
CSB-MP429659YAM
CSB-EP429659YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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