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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella canis ATP synthase gamma chain (atpG) CSB-YP430322BPH
CSB-EP430322BPH
CSB-BP430322BPH
CSB-MP430322BPH
CSB-EP430322BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis RNA pyrophosphohydrolase (rppH) CSB-YP430323BPH
CSB-EP430323BPH
CSB-BP430323BPH
CSB-MP430323BPH
CSB-EP430323BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis tRNA (guanine-N (1)-)-methyltransferase CSB-YP430324BPH
CSB-EP430324BPH
CSB-BP430324BPH
CSB-MP430324BPH
CSB-EP430324BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis UPF0178 protein BCAN_A2024 (BCAN_A2024) CSB-YP430325BPH
CSB-EP430325BPH
CSB-BP430325BPH
CSB-MP430325BPH
CSB-EP430325BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP430326BPH
CSB-EP430326BPH
CSB-BP430326BPH
CSB-MP430326BPH
CSB-EP430326BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP430327BPH
CSB-EP430327BPH
CSB-BP430327BPH
CSB-MP430327BPH
CSB-EP430327BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Tryptophan synthase alpha chain (trpA) CSB-YP430328BPH
CSB-EP430328BPH
CSB-BP430328BPH
CSB-MP430328BPH
CSB-EP430328BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Chaperone protein DnaJ (dnaJ) CSB-YP430329BPH
CSB-EP430329BPH
CSB-BP430329BPH
CSB-MP430329BPH
CSB-EP430329BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis tRNA pseudouridine synthase B (truB) CSB-YP430330BPH
CSB-EP430330BPH
CSB-BP430330BPH
CSB-MP430330BPH
CSB-EP430330BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP430331BPH
CSB-EP430331BPH
CSB-BP430331BPH
CSB-MP430331BPH
CSB-EP430331BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 30S ribosomal protein S20 (rpsT) CSB-YP430332BPH
CSB-EP430332BPH
CSB-BP430332BPH
CSB-MP430332BPH
CSB-EP430332BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP430333BPH
CSB-EP430333BPH
CSB-BP430333BPH
CSB-MP430333BPH
CSB-EP430333BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 50S ribosomal protein L13 (rplM) CSB-YP430334BPH
CSB-EP430334BPH
CSB-BP430334BPH
CSB-MP430334BPH
CSB-EP430334BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP430335BPH1
CSB-EP430335BPH1
CSB-BP430335BPH1
CSB-MP430335BPH1
CSB-EP430335BPH1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP430336BPH
CSB-EP430336BPH
CSB-BP430336BPH
CSB-MP430336BPH
CSB-EP430336BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Sec-independent protein translocase protein TatA (tatA), partial CSB-YP430337BPH
CSB-EP430337BPH
CSB-BP430337BPH
CSB-MP430337BPH
CSB-EP430337BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Glutamate--tRNA ligase 1 (gltX1) CSB-YP430338BPH
CSB-EP430338BPH
CSB-BP430338BPH
CSB-MP430338BPH
CSB-EP430338BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Polar-differentiation response regulator divK (divK) CSB-YP430339BPH
CSB-EP430339BPH
CSB-BP430339BPH
CSB-MP430339BPH
CSB-EP430339BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Putative nickel-responsive regulator (BCAN_B0819) CSB-YP430340BPH
CSB-EP430340BPH
CSB-BP430340BPH
CSB-MP430340BPH
CSB-EP430340BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Alanine racemase (alr) CSB-YP430341BPH
CSB-EP430341BPH
CSB-BP430341BPH
CSB-MP430341BPH
CSB-EP430341BPH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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