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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cuscuta obtusiflora Photosystem II D2 protein (psbD), partial CSB-YP428900CXS1
CSB-EP428900CXS1
CSB-BP428900CXS1
CSB-MP428900CXS1
CSB-EP428900CXS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora Apocytochrome f (petA), partial CSB-YP428901CXS1
CSB-EP428901CXS1
CSB-BP428901CXS1
CSB-MP428901CXS1
CSB-EP428901CXS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora Protein psbN (psbN), partial CSB-YP428902CXS
CSB-EP428902CXS
CSB-BP428902CXS
CSB-MP428902CXS
CSB-EP428902CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta obtusiflora 30S ribosomal protein S15, plastid (rps15) CSB-YP428903CXS
CSB-EP428903CXS
CSB-BP428903CXS
CSB-MP428903CXS
CSB-EP428903CXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Uncharacterized protein C11orf91 homolog CSB-YP428904BO
CSB-EP428904BO
CSB-BP428904BO
CSB-MP428904BO
CSB-EP428904BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Structure-specific endonuclease subunit SLX1 homolog (giyd-1) CSB-YP428905CXX
CSB-EP428905CXX
CSB-BP428905CXX
CSB-MP428905CXX
CSB-EP428905CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable eukaryotic translation initiation factor 3 subunit G (eif-3.G.1) CSB-YP428906CXX
CSB-EP428906CXX
CSB-BP428906CXX
CSB-MP428906CXX
CSB-EP428906CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotinic receptor-associated protein 1 (nra-1), partial CSB-YP428907CXX
CSB-EP428907CXX
CSB-BP428907CXX
CSB-MP428907CXX
CSB-EP428907CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable medium-chain specific acyl-CoA dehydrogenase 2, mitochondrial (CBG00953) CSB-YP428908CXX
CSB-EP428908CXX
CSB-BP428908CXX
CSB-MP428908CXX
CSB-EP428908CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulator of telomere elongation helicase 1 homolog (rtel-1), partial CSB-YP428909CXX
CSB-EP428909CXX
CSB-BP428909CXX
CSB-MP428909CXX
CSB-EP428909CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (guanine-N (7)-)-methyltransferase CSB-YP428910CXX
CSB-EP428910CXX
CSB-BP428910CXX
CSB-MP428910CXX
CSB-EP428910CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein dct-5 (dct-5), partial CSB-YP428911CXX1
CSB-EP428911CXX1
CSB-BP428911CXX1
CSB-MP428911CXX1
CSB-EP428911CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chondroitin proteoglycan 9 (cpg-9) CSB-YP428912CXX
CSB-EP428912CXX
CSB-BP428912CXX
CSB-MP428912CXX
CSB-EP428912CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant E3 ubiquitin-protein ligase hrd-like protein 1 (hrdl-1), partial CSB-YP428913CXX
CSB-EP428913CXX
CSB-BP428913CXX
CSB-MP428913CXX
CSB-EP428913CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-aspartate oxidase 1 (CBG04460) CSB-YP428914CXX
CSB-EP428914CXX
CSB-BP428914CXX
CSB-MP428914CXX
CSB-EP428914CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tat-binding homolog 7 (lex-1), partial CSB-YP428915CXX
CSB-EP428915CXX
CSB-BP428915CXX
CSB-MP428915CXX
CSB-EP428915CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chondroitin proteoglycan 8 (cpg-8) CSB-YP428916CXX
CSB-EP428916CXX
CSB-BP428916CXX
CSB-MP428916CXX
CSB-EP428916CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histone H3-like centromeric protein cpar-1 (cpar-1) CSB-YP428917CXX
CSB-EP428917CXX
CSB-BP428917CXX
CSB-MP428917CXX
CSB-EP428917CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetratricopeptide repeat protein 30 homolog (dyf-1), partial CSB-YP428918CXX
CSB-EP428918CXX
CSB-BP428918CXX
CSB-MP428918CXX
CSB-EP428918CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ubiquitin thioesterase otubain-like (otub-1) CSB-YP428919CXX
CSB-EP428919CXX
CSB-BP428919CXX
CSB-MP428919CXX
CSB-EP428919CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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