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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella arizonae GTPase obg (obg) CSB-YP430402STE
CSB-EP430402STE
CSB-BP430402STE
CSB-MP430402STE
CSB-EP430402STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0260 protein ycgN (ycgN) CSB-YP430403STE
CSB-EP430403STE
CSB-BP430403STE
CSB-MP430403STE
CSB-EP430403STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Probable intracellular septation protein A (yciB), partial CSB-YP430404STE1
CSB-EP430404STE1
CSB-BP430404STE1
CSB-MP430404STE1
CSB-EP430404STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Deoxyguanosinetriphosphate triphosphohydrolase (dgt), partial CSB-YP430405STE
CSB-EP430405STE
CSB-BP430405STE
CSB-MP430405STE
CSB-EP430405STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP430406STE
CSB-EP430406STE
CSB-BP430406STE
CSB-MP430406STE
CSB-EP430406STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae D-tagatose-1,6-bisphosphate aldolase subunit KbaY (kbaY) CSB-YP430407STE
CSB-EP430407STE
CSB-BP430407STE
CSB-MP430407STE
CSB-EP430407STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0597 protein yhaM (yhaM) CSB-YP430408STE
CSB-EP430408STE
CSB-BP430408STE
CSB-MP430408STE
CSB-EP430408STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 30S ribosomal protein S21 (rpsU) CSB-YP430409STE
CSB-EP430409STE
CSB-BP430409STE
CSB-MP430409STE
CSB-EP430409STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP430410STE
CSB-EP430410STE
CSB-BP430410STE
CSB-MP430410STE
CSB-EP430410STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae DNA gyrase inhibitor YacG (yacG) CSB-YP430411STE
CSB-EP430411STE
CSB-BP430411STE
CSB-MP430411STE
CSB-EP430411STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP430412STE
CSB-EP430412STE
CSB-BP430412STE
CSB-MP430412STE
CSB-EP430412STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Glutathione-regulated potassium-efflux system protein kefC (kefC), partial CSB-YP430413STE
CSB-EP430413STE
CSB-BP430413STE
CSB-MP430413STE
CSB-EP430413STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Anti-adapter protein iraD (iraD) CSB-YP430414STE
CSB-EP430414STE
CSB-BP430414STE
CSB-MP430414STE
CSB-EP430414STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Putative Holliday junction resolvase (yqgF) CSB-YP430415STE
CSB-EP430415STE
CSB-BP430415STE
CSB-MP430415STE
CSB-EP430415STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Na (+)/H (+) antiporter nhaA, partial CSB-YP430416STE
CSB-EP430416STE
CSB-BP430416STE
CSB-MP430416STE
CSB-EP430416STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP430417STE
CSB-EP430417STE
CSB-BP430417STE
CSB-MP430417STE
CSB-EP430417STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Nickel/cobalt efflux system rcnA (rcnA), partial CSB-YP430418STE1
CSB-EP430418STE1
CSB-BP430418STE1
CSB-MP430418STE1
CSB-EP430418STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Spermidine export protein MdtJ (mdtJ), partial CSB-YP430419STE1
CSB-EP430419STE1
CSB-BP430419STE1
CSB-MP430419STE1
CSB-EP430419STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Phosphoglycerol transferase I (mdoB), partial CSB-YP430420STE1
CSB-EP430420STE1
CSB-BP430420STE1
CSB-MP430420STE1
CSB-EP430420STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP430421STE
CSB-EP430421STE
CSB-BP430421STE
CSB-MP430421STE
CSB-EP430421STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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