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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium phytofermentans 50S ribosomal protein L27 (rpmA) CSB-YP430202CWZ
CSB-EP430202CWZ
CSB-BP430202CWZ
CSB-MP430202CWZ
CSB-EP430202CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Ribonuclease Y (rny), partial CSB-YP430203CWZ
CSB-EP430203CWZ
CSB-BP430203CWZ
CSB-MP430203CWZ
CSB-EP430203CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Phosphate propanoyltransferase (pduL) CSB-YP430204CWZ
CSB-EP430204CWZ
CSB-BP430204CWZ
CSB-MP430204CWZ
CSB-EP430204CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP430205CWZ
CSB-EP430205CWZ
CSB-BP430205CWZ
CSB-MP430205CWZ
CSB-EP430205CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Glutamyl-tRNA reductase 1 (hemA1) CSB-YP430206CWZ
CSB-EP430206CWZ
CSB-BP430206CWZ
CSB-MP430206CWZ
CSB-EP430206CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP430207CWZ
CSB-EP430207CWZ
CSB-BP430207CWZ
CSB-MP430207CWZ
CSB-EP430207CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Chromosomal replication initiator protein DnaA (dnaA) CSB-YP430208CWZ
CSB-EP430208CWZ
CSB-BP430208CWZ
CSB-MP430208CWZ
CSB-EP430208CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans UPF0133 protein Cphy_0047 (Cphy_0047) CSB-YP430209CWZ
CSB-EP430209CWZ
CSB-BP430209CWZ
CSB-MP430209CWZ
CSB-EP430209CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Peptidyl-tRNA hydrolase (pth) CSB-YP430210CWZ
CSB-EP430210CWZ
CSB-BP430210CWZ
CSB-MP430210CWZ
CSB-EP430210CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 30S ribosomal protein S7 (rpsG) CSB-YP430211CWZ
CSB-EP430211CWZ
CSB-BP430211CWZ
CSB-MP430211CWZ
CSB-EP430211CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Probable DNA repair protein Cphy_1728 (Cphy_1728) CSB-YP430212CWZ
CSB-EP430212CWZ
CSB-BP430212CWZ
CSB-MP430212CWZ
CSB-EP430212CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 60 kDa chaperonin (groL), partial CSB-YP430213CWZ
CSB-EP430213CWZ
CSB-BP430213CWZ
CSB-MP430213CWZ
CSB-EP430213CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans UvrABC system protein C (uvrC), partial CSB-YP430214CWZ
CSB-EP430214CWZ
CSB-BP430214CWZ
CSB-MP430214CWZ
CSB-EP430214CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP430215CWZ
CSB-EP430215CWZ
CSB-BP430215CWZ
CSB-MP430215CWZ
CSB-EP430215CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP430216CWZ
CSB-EP430216CWZ
CSB-BP430216CWZ
CSB-MP430216CWZ
CSB-EP430216CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA sulfurtransferase (thiI) CSB-YP430217STN
CSB-EP430217STN
CSB-BP430217STN
CSB-MP430217STN
CSB-EP430217STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP430218STN
CSB-EP430218STN
CSB-BP430218STN
CSB-MP430218STN
CSB-EP430218STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP430219STN
CSB-EP430219STN
CSB-BP430219STN
CSB-MP430219STN
CSB-EP430219STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP430220STN
CSB-EP430220STN
CSB-BP430220STN
CSB-MP430220STN
CSB-EP430220STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP430221STN
CSB-EP430221STN
CSB-BP430221STN
CSB-MP430221STN
CSB-EP430221STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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