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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia multivorans Ribosome-binding factor A (rbfA) CSB-YP429901BPS
CSB-EP429901BPS
CSB-BP429901BPS
CSB-MP429901BPS
CSB-EP429901BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Flagellar hook-basal body complex protein FliE (fliE) CSB-YP429902BPS
CSB-EP429902BPS
CSB-BP429902BPS
CSB-MP429902BPS
CSB-EP429902BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Protease HtpX homolog (htpX), partial CSB-YP429903BPS1
CSB-EP429903BPS1
CSB-BP429903BPS1
CSB-MP429903BPS1
CSB-EP429903BPS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP429904BPS
CSB-EP429904BPS
CSB-BP429904BPS
CSB-MP429904BPS
CSB-EP429904BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L11 (rplK) CSB-YP429905BPS
CSB-EP429905BPS
CSB-BP429905BPS
CSB-MP429905BPS
CSB-EP429905BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S12 (rpsL) CSB-YP429906BPS
CSB-EP429906BPS
CSB-BP429906BPS
CSB-MP429906BPS
CSB-EP429906BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S17 (rpsQ) CSB-YP429907BPS
CSB-EP429907BPS
CSB-BP429907BPS
CSB-MP429907BPS
CSB-EP429907BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L30 (rpmD) CSB-YP429908BPS
CSB-EP429908BPS
CSB-BP429908BPS
CSB-MP429908BPS
CSB-EP429908BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S13 (rpsM) CSB-YP429909BPS
CSB-EP429909BPS
CSB-BP429909BPS
CSB-MP429909BPS
CSB-EP429909BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Ribosome maturation factor RimM (rimM) CSB-YP429910BPS
CSB-EP429910BPS
CSB-BP429910BPS
CSB-MP429910BPS
CSB-EP429910BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Biotin synthase (bioB) CSB-YP429911BPS
CSB-EP429911BPS
CSB-BP429911BPS
CSB-MP429911BPS
CSB-EP429911BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans C4-dicarboxylate transport protein (dctA), partial CSB-YP429912BPS
CSB-EP429912BPS
CSB-BP429912BPS
CSB-MP429912BPS
CSB-EP429912BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP429913BPS
CSB-EP429913BPS
CSB-BP429913BPS
CSB-MP429913BPS
CSB-EP429913BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP429914BPS
CSB-EP429914BPS
CSB-BP429914BPS
CSB-MP429914BPS
CSB-EP429914BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Urease subunit alpha (ureC), partial CSB-YP429915BPS
CSB-EP429915BPS
CSB-BP429915BPS
CSB-MP429915BPS
CSB-EP429915BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP429916BPS
CSB-EP429916BPS
CSB-BP429916BPS
CSB-MP429916BPS
CSB-EP429916BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UPF0246 protein Bmul_1054/BMULJ_02209 (Bmul_1054, BMULJ_02209) CSB-YP429917BPS
CSB-EP429917BPS
CSB-BP429917BPS
CSB-MP429917BPS
CSB-EP429917BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Heat-inducible transcription repressor HrcA (hrcA) CSB-YP429918BPS
CSB-EP429918BPS
CSB-BP429918BPS
CSB-MP429918BPS
CSB-EP429918BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UPF0234 protein Bmul_0741/BMULJ_02519 (Bmul_0741, BMULJ_02519) CSB-YP429919BPS
CSB-EP429919BPS
CSB-BP429919BPS
CSB-MP429919BPS
CSB-EP429919BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP429920BPS
CSB-EP429920BPS
CSB-BP429920BPS
CSB-MP429920BPS
CSB-EP429920BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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