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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP429961PZC
CSB-EP429961PZC
CSB-BP429961PZC
CSB-MP429961PZC
CSB-EP429961PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase (acsF) CSB-YP429962PZC
CSB-EP429962PZC
CSB-BP429962PZC
CSB-MP429962PZC
CSB-EP429962PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamyl-tRNA reductase (hemA) CSB-YP429963PZC
CSB-EP429963PZC
CSB-BP429963PZC
CSB-MP429963PZC
CSB-EP429963PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Threonine--tRNA ligase (thrS), partial CSB-YP429964PZC
CSB-EP429964PZC
CSB-BP429964PZC
CSB-MP429964PZC
CSB-EP429964PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP429965PZC
CSB-EP429965PZC
CSB-BP429965PZC
CSB-MP429965PZC
CSB-EP429965PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP429966PZC
CSB-EP429966PZC
CSB-BP429966PZC
CSB-MP429966PZC
CSB-EP429966PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP429967PZC
CSB-EP429967PZC
CSB-BP429967PZC
CSB-MP429967PZC
CSB-EP429967PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP synthase epsilon chain (atpC) CSB-YP429968PZC
CSB-EP429968PZC
CSB-BP429968PZC
CSB-MP429968PZC
CSB-EP429968PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S7 (rpsG) CSB-YP429969PZC
CSB-EP429969PZC
CSB-BP429969PZC
CSB-MP429969PZC
CSB-EP429969PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L17 (rplQ) CSB-YP429970PZC
CSB-EP429970PZC
CSB-BP429970PZC
CSB-MP429970PZC
CSB-EP429970PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S8 (rpsH) CSB-YP429971PZC
CSB-EP429971PZC
CSB-BP429971PZC
CSB-MP429971PZC
CSB-EP429971PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L22 (rplV) CSB-YP429972PZC
CSB-EP429972PZC
CSB-BP429972PZC
CSB-MP429972PZC
CSB-EP429972PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP429973PZC
CSB-EP429973PZC
CSB-BP429973PZC
CSB-MP429973PZC
CSB-EP429973PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosome maturation factor RimM (rimM) CSB-YP429974PZC
CSB-EP429974PZC
CSB-BP429974PZC
CSB-MP429974PZC
CSB-EP429974PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UvrABC system protein B (uvrB), partial CSB-YP429975PZC
CSB-EP429975PZC
CSB-BP429975PZC
CSB-MP429975PZC
CSB-EP429975PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Aspartate--tRNA ligase (aspS), partial CSB-YP429976PZC
CSB-EP429976PZC
CSB-BP429976PZC
CSB-MP429976PZC
CSB-EP429976PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Enolase (eno) CSB-YP429977PZC
CSB-EP429977PZC
CSB-BP429977PZC
CSB-MP429977PZC
CSB-EP429977PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein H (psbH), partial CSB-YP429978PZC1
CSB-EP429978PZC1
CSB-BP429978PZC1
CSB-MP429978PZC1
CSB-EP429978PZC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP429979PZC
CSB-EP429979PZC
CSB-BP429979PZC
CSB-MP429979PZC
CSB-EP429979PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit J CSB-YP429980PZC
CSB-EP429980PZC
CSB-BP429980PZC
CSB-MP429980PZC
CSB-EP429980PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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