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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Delftia acidovorans RNA pyrophosphohydrolase (rppH) CSB-YP430902DHV
CSB-EP430902DHV
CSB-BP430902DHV
CSB-MP430902DHV
CSB-EP430902DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L22 (rplV) CSB-YP430903DHV
CSB-EP430903DHV
CSB-BP430903DHV
CSB-MP430903DHV
CSB-EP430903DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans ATP synthase gamma chain (atpG) CSB-YP430904DHV
CSB-EP430904DHV
CSB-BP430904DHV
CSB-MP430904DHV
CSB-EP430904DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Cell division topological specificity factor (minE) CSB-YP430905DHV
CSB-EP430905DHV
CSB-BP430905DHV
CSB-MP430905DHV
CSB-EP430905DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L7/L12 (rplL) CSB-YP430906DHV
CSB-EP430906DHV
CSB-BP430906DHV
CSB-MP430906DHV
CSB-EP430906DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Glycerol kinase (glpK) CSB-YP430907DHV
CSB-EP430907DHV
CSB-BP430907DHV
CSB-MP430907DHV
CSB-EP430907DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP430908DHV
CSB-EP430908DHV
CSB-BP430908DHV
CSB-MP430908DHV
CSB-EP430908DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Non-canonical purine NTP pyrophosphatase (Daci_2032) CSB-YP430909DHV
CSB-EP430909DHV
CSB-BP430909DHV
CSB-MP430909DHV
CSB-EP430909DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Urease accessory protein UreF (ureF) CSB-YP430910DHV
CSB-EP430910DHV
CSB-BP430910DHV
CSB-MP430910DHV
CSB-EP430910DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP430911DHV
CSB-EP430911DHV
CSB-BP430911DHV
CSB-MP430911DHV
CSB-EP430911DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans D-alanine--D-alanine ligase (ddl) CSB-YP430912DHV
CSB-EP430912DHV
CSB-BP430912DHV
CSB-MP430912DHV
CSB-EP430912DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP430913DHV
CSB-EP430913DHV
CSB-BP430913DHV
CSB-MP430913DHV
CSB-EP430913DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP430914DHV
CSB-EP430914DHV
CSB-BP430914DHV
CSB-MP430914DHV
CSB-EP430914DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP430915DHV
CSB-EP430915DHV
CSB-BP430915DHV
CSB-MP430915DHV
CSB-EP430915DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans S-adenosylmethionine synthase (metK) CSB-YP430916DHV
CSB-EP430916DHV
CSB-BP430916DHV
CSB-MP430916DHV
CSB-EP430916DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Acetylglutamate kinase (argB) CSB-YP430917DHV
CSB-EP430917DHV
CSB-BP430917DHV
CSB-MP430917DHV
CSB-EP430917DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP430918DHV
CSB-EP430918DHV
CSB-BP430918DHV
CSB-MP430918DHV
CSB-EP430918DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Integration host factor subunit alpha (ihfA) CSB-YP430919DHV
CSB-EP430919DHV
CSB-BP430919DHV
CSB-MP430919DHV
CSB-EP430919DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Ferredoxin--NADP reductase (Daci_4658) CSB-YP430920DHV
CSB-EP430920DHV
CSB-BP430920DHV
CSB-MP430920DHV
CSB-EP430920DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Biotin synthase (bioB) CSB-YP430921AYS
CSB-EP430921AYS
CSB-BP430921AYS
CSB-MP430921AYS
CSB-EP430921AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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