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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella paratyphi B Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP430502STF1
CSB-EP430502STF1
CSB-BP430502STF1
CSB-MP430502STF1
CSB-EP430502STF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Chaperone protein DnaJ (dnaJ) CSB-YP430503CXH
CSB-EP430503CXH
CSB-BP430503CXH
CSB-MP430503CXH
CSB-EP430503CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii SsrA-binding protein (smpB) CSB-YP430504CXH
CSB-EP430504CXH
CSB-BP430504CXH
CSB-MP430504CXH
CSB-EP430504CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L35 (rpmI) CSB-YP430505CXH
CSB-EP430505CXH
CSB-BP430505CXH
CSB-MP430505CXH
CSB-EP430505CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP430506CXH
CSB-EP430506CXH
CSB-BP430506CXH
CSB-MP430506CXH
CSB-EP430506CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Cysteine--tRNA ligase (cysS) CSB-YP430507CXH
CSB-EP430507CXH
CSB-BP430507CXH
CSB-MP430507CXH
CSB-EP430507CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP430508CXH
CSB-EP430508CXH
CSB-BP430508CXH
CSB-MP430508CXH
CSB-EP430508CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP430509CXH
CSB-EP430509CXH
CSB-BP430509CXH
CSB-MP430509CXH
CSB-EP430509CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UPF0761 membrane protein COXBURSA331_A1765 (COXBURSA331_A1765), partial CSB-YP430510CXH1
CSB-EP430510CXH1
CSB-BP430510CXH1
CSB-MP430510CXH1
CSB-EP430510CXH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP-dependent protease subunit HslV (hslV) CSB-YP430511CXH
CSB-EP430511CXH
CSB-BP430511CXH
CSB-MP430511CXH
CSB-EP430511CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP430512CXH
CSB-EP430512CXH
CSB-BP430512CXH
CSB-MP430512CXH
CSB-EP430512CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Protein MraZ (mraZ) CSB-YP430513CXH
CSB-EP430513CXH
CSB-BP430513CXH
CSB-MP430513CXH
CSB-EP430513CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP430514CXH
CSB-EP430514CXH
CSB-BP430514CXH
CSB-MP430514CXH
CSB-EP430514CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP430515CXH
CSB-EP430515CXH
CSB-BP430515CXH
CSB-MP430515CXH
CSB-EP430515CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP430516CXH
CSB-EP430516CXH
CSB-BP430516CXH
CSB-MP430516CXH
CSB-EP430516CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UPF0289 protein COXBURSA331_A0242 (COXBURSA331_A0242) CSB-YP430517CXH
CSB-EP430517CXH
CSB-BP430517CXH
CSB-MP430517CXH
CSB-EP430517CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L10 (rplJ) CSB-YP430518CXH
CSB-EP430518CXH
CSB-BP430518CXH
CSB-MP430518CXH
CSB-EP430518CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L3 (rplC) CSB-YP430519CXH
CSB-EP430519CXH
CSB-BP430519CXH
CSB-MP430519CXH
CSB-EP430519CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L22 (rplV) CSB-YP430520CXH
CSB-EP430520CXH
CSB-BP430520CXH
CSB-MP430520CXH
CSB-EP430520CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii CCA-adding enzyme (cca) CSB-YP430521CXH
CSB-EP430521CXH
CSB-BP430521CXH
CSB-MP430521CXH
CSB-EP430521CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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