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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Physcomitrella patens subsp. patens CASP-like protein PHYPADRAFT_66946 (PHYPADRAFT_66946), partial CSB-YP431502EVD1
CSB-EP431502EVD1
CSB-BP431502EVD1
CSB-MP431502EVD1
CSB-EP431502EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens CASP-like protein PHYPADRAFT_164940 (PHYPADRAFT_164940), partial CSB-YP431503EVD1
CSB-EP431503EVD1
CSB-BP431503EVD1
CSB-MP431503EVD1
CSB-EP431503EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens CASP-like protein PHYPADRAFT_108355 (PHYPADRAFT_108355), partial CSB-YP431504EVD1
CSB-EP431504EVD1
CSB-BP431504EVD1
CSB-MP431504EVD1
CSB-EP431504EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus californicus Mu-conotoxin-like CalTx 12.2.1E CSB-YP431505DVU
CSB-EP431505DVU
CSB-BP431505DVU
CSB-MP431505DVU
CSB-EP431505DVU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis tRNA (guanine-N (7)-)-methyltransferase B CSB-YP431506XBF
CSB-EP431506XBF
CSB-BP431506XBF
CSB-MP431506XBF
CSB-EP431506XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis UPF0518 protein 36947 (36947), partial CSB-YP431507MOD
CSB-EP431507MOD
CSB-BP431507MOD
CSB-MP431507MOD
CSB-EP431507MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Methylmalonate semialdehyde dehydrogenase [acylating] 1 (iolA1) CSB-YP431508BON
CSB-EP431508BON
CSB-BP431508BON
CSB-MP431508BON
CSB-EP431508BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP431509BON
CSB-EP431509BON
CSB-BP431509BON
CSB-MP431509BON
CSB-EP431509BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP431510BON
CSB-EP431510BON
CSB-BP431510BON
CSB-MP431510BON
CSB-EP431510BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP431511BON
CSB-EP431511BON
CSB-BP431511BON
CSB-MP431511BON
CSB-EP431511BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable butyrate kinase (buk) CSB-YP431512BON
CSB-EP431512BON
CSB-BP431512BON
CSB-MP431512BON
CSB-EP431512BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Putative metal-dependent hydrolase BcerKBAB4_2443 (BcerKBAB4_2443) CSB-YP431513BON
CSB-EP431513BON
CSB-BP431513BON
CSB-MP431513BON
CSB-EP431513BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Kynurenine formamidase (kynB) CSB-YP431514BON
CSB-EP431514BON
CSB-BP431514BON
CSB-MP431514BON
CSB-EP431514BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Putative phosphotransferase BcerKBAB4_4147 (BcerKBAB4_4147) CSB-YP431515BON
CSB-EP431515BON
CSB-BP431515BON
CSB-MP431515BON
CSB-EP431515BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP431516BON
CSB-EP431516BON
CSB-BP431516BON
CSB-MP431516BON
CSB-EP431516BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0297 protein BcerKBAB4_4234 (BcerKBAB4_4234) CSB-YP431517BON
CSB-EP431517BON
CSB-BP431517BON
CSB-MP431517BON
CSB-EP431517BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Lactate utilization protein A 1 (lutA1) CSB-YP431518BON
CSB-EP431518BON
CSB-BP431518BON
CSB-MP431518BON
CSB-EP431518BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Aspartate--tRNA ligase (aspS), partial CSB-YP431519BON
CSB-EP431519BON
CSB-BP431519BON
CSB-MP431519BON
CSB-EP431519BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP431520BON
CSB-EP431520BON
CSB-BP431520BON
CSB-MP431520BON
CSB-EP431520BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0758 protein BcerKBAB4_4299 (BcerKBAB4_4299) CSB-YP431521BON
CSB-EP431521BON
CSB-BP431521BON
CSB-MP431521BON
CSB-EP431521BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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