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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Coxiella burnetii UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP430162DXO
CSB-EP430162DXO
CSB-BP430162DXO
CSB-MP430162DXO
CSB-EP430162DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Probable Fe (2+)-trafficking protein CSB-YP430163DXO
CSB-EP430163DXO
CSB-BP430163DXO
CSB-MP430163DXO
CSB-EP430163DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA ligase (ligA), partial CSB-YP430164DXO
CSB-EP430164DXO
CSB-BP430164DXO
CSB-MP430164DXO
CSB-EP430164DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S13 (rpsM) CSB-YP430165DXO
CSB-EP430165DXO
CSB-BP430165DXO
CSB-MP430165DXO
CSB-EP430165DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S8 (rpsH) CSB-YP430166DXO
CSB-EP430166DXO
CSB-BP430166DXO
CSB-MP430166DXO
CSB-EP430166DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L2 (rplB) CSB-YP430167DXO
CSB-EP430167DXO
CSB-BP430167DXO
CSB-MP430167DXO
CSB-EP430167DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S12 (rpsL) CSB-YP430168DXO
CSB-EP430168DXO
CSB-BP430168DXO
CSB-MP430168DXO
CSB-EP430168DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L7/L12 (rplL) CSB-YP430169DXO
CSB-EP430169DXO
CSB-BP430169DXO
CSB-MP430169DXO
CSB-EP430169DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii S-adenosylmethionine synthase (metK) CSB-YP430170DXO
CSB-EP430170DXO
CSB-BP430170DXO
CSB-MP430170DXO
CSB-EP430170DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP430171DXO
CSB-EP430171DXO
CSB-BP430171DXO
CSB-MP430171DXO
CSB-EP430171DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L19 (rplS) CSB-YP430172DXO
CSB-EP430172DXO
CSB-BP430172DXO
CSB-MP430172DXO
CSB-EP430172DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Isoleucine--tRNA ligase (ileS), partial CSB-YP430173DXO
CSB-EP430173DXO
CSB-BP430173DXO
CSB-MP430173DXO
CSB-EP430173DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP430174DXO
CSB-EP430174DXO
CSB-BP430174DXO
CSB-MP430174DXO
CSB-EP430174DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP430175DXO
CSB-EP430175DXO
CSB-BP430175DXO
CSB-MP430175DXO
CSB-EP430175DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Probable transcriptional regulatory protein CBUD_0422 (CBUD_0422) CSB-YP430176DXO
CSB-EP430176DXO
CSB-BP430176DXO
CSB-MP430176DXO
CSB-EP430176DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA repair protein recO (recO) CSB-YP430177DXO
CSB-EP430177DXO
CSB-BP430177DXO
CSB-MP430177DXO
CSB-EP430177DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Peptide chain release factor 3 (prfC), partial CSB-YP430178DXO
CSB-EP430178DXO
CSB-BP430178DXO
CSB-MP430178DXO
CSB-EP430178DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L9 (rplI) CSB-YP430179DXO
CSB-EP430179DXO
CSB-BP430179DXO
CSB-MP430179DXO
CSB-EP430179DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Translation initiation factor IF-1 (infA) CSB-YP430180DXO
CSB-EP430180DXO
CSB-BP430180DXO
CSB-MP430180DXO
CSB-EP430180DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Alanine--tRNA ligase (alaS), partial CSB-YP430181DXO
CSB-EP430181DXO
CSB-BP430181DXO
CSB-MP430181DXO
CSB-EP430181DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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