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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis bv. Antiqua 30S ribosomal protein S18 (rpsR) CSB-YP430562YAM
CSB-EP430562YAM
CSB-BP430562YAM
CSB-MP430562YAM
CSB-EP430562YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 60 kDa chaperonin (groL), partial CSB-YP430563YAM
CSB-EP430563YAM
CSB-BP430563YAM
CSB-MP430563YAM
CSB-EP430563YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP430564YAM
CSB-EP430564YAM
CSB-BP430564YAM
CSB-MP430564YAM
CSB-EP430564YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Arginine--tRNA ligase (argS), partial CSB-YP430565YAM
CSB-EP430565YAM
CSB-BP430565YAM
CSB-MP430565YAM
CSB-EP430565YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Succinylglutamate desuccinylase (astE) CSB-YP430566YAM
CSB-EP430566YAM
CSB-BP430566YAM
CSB-MP430566YAM
CSB-EP430566YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP430567YAM
CSB-EP430567YAM
CSB-BP430567YAM
CSB-MP430567YAM
CSB-EP430567YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua N-acetylmannosamine kinase (nanK) CSB-YP430568YAM
CSB-EP430568YAM
CSB-BP430568YAM
CSB-MP430568YAM
CSB-EP430568YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L11 (rplK) CSB-YP430569YAM
CSB-EP430569YAM
CSB-BP430569YAM
CSB-MP430569YAM
CSB-EP430569YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Trigger factor (tig) CSB-YP430570YAM
CSB-EP430570YAM
CSB-BP430570YAM
CSB-MP430570YAM
CSB-EP430570YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP430571YAM
CSB-EP430571YAM
CSB-BP430571YAM
CSB-MP430571YAM
CSB-EP430571YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Chaperone protein DnaK (dnaK), partial CSB-YP430572YAM
CSB-EP430572YAM
CSB-BP430572YAM
CSB-MP430572YAM
CSB-EP430572YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Phosphopentomutase (deoB) CSB-YP430573YAM
CSB-EP430573YAM
CSB-BP430573YAM
CSB-MP430573YAM
CSB-EP430573YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP430574YAM
CSB-EP430574YAM
CSB-BP430574YAM
CSB-MP430574YAM
CSB-EP430574YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP430575YAM
CSB-EP430575YAM
CSB-BP430575YAM
CSB-MP430575YAM
CSB-EP430575YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Peptidase T (pepT) CSB-YP430576YAM
CSB-EP430576YAM
CSB-BP430576YAM
CSB-MP430576YAM
CSB-EP430576YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Alanine--tRNA ligase (alaS), partial CSB-YP430577YAM
CSB-EP430577YAM
CSB-BP430577YAM
CSB-MP430577YAM
CSB-EP430577YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua tRNA pseudouridine synthase D (truD) CSB-YP430578YAM
CSB-EP430578YAM
CSB-BP430578YAM
CSB-MP430578YAM
CSB-EP430578YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Protein MraZ (mraZ) CSB-YP430579YAM
CSB-EP430579YAM
CSB-BP430579YAM
CSB-MP430579YAM
CSB-EP430579YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Enolase (eno) CSB-YP430580YAM
CSB-EP430580YAM
CSB-BP430580YAM
CSB-MP430580YAM
CSB-EP430580YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Vitamin B12-binding protein (btuF) CSB-YP430581YAM
CSB-EP430581YAM
CSB-BP430581YAM
CSB-MP430581YAM
CSB-EP430581YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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