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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP430242STN
CSB-EP430242STN
CSB-BP430242STN
CSB-MP430242STN
CSB-EP430242STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP430243STN
CSB-EP430243STN
CSB-BP430243STN
CSB-MP430243STN
CSB-EP430243STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica S-ribosylhomocysteine lyase (luxS) CSB-YP430244STN
CSB-EP430244STN
CSB-BP430244STN
CSB-MP430244STN
CSB-EP430244STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Primosomal replication protein n (priB) CSB-YP430245STN
CSB-EP430245STN
CSB-BP430245STN
CSB-MP430245STN
CSB-EP430245STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica PKHD-type hydroxylase Sbal195_0750 (Sbal195_0750) CSB-YP430246STN
CSB-EP430246STN
CSB-BP430246STN
CSB-MP430246STN
CSB-EP430246STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Catalase-peroxidase (katG), partial CSB-YP430247STN
CSB-EP430247STN
CSB-BP430247STN
CSB-MP430247STN
CSB-EP430247STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Non-canonical purine NTP phosphatase (Sbal195_3676) CSB-YP430248STN
CSB-EP430248STN
CSB-BP430248STN
CSB-MP430248STN
CSB-EP430248STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L35 (rpmI) CSB-YP430249STN
CSB-EP430249STN
CSB-BP430249STN
CSB-MP430249STN
CSB-EP430249STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA (cmo5U34)-methyltransferase CSB-YP430250STN
CSB-EP430250STN
CSB-BP430250STN
CSB-MP430250STN
CSB-EP430250STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Chaperone protein hscA homolog (hscA), partial CSB-YP430251STN
CSB-EP430251STN
CSB-BP430251STN
CSB-MP430251STN
CSB-EP430251STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP430252STN
CSB-EP430252STN
CSB-BP430252STN
CSB-MP430252STN
CSB-EP430252STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Uridine kinase (udk) CSB-YP430253STN
CSB-EP430253STN
CSB-BP430253STN
CSB-MP430253STN
CSB-EP430253STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP430254STN
CSB-EP430254STN
CSB-BP430254STN
CSB-MP430254STN
CSB-EP430254STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal protein S6 modification protein 2 (rimK2) CSB-YP430255STN
CSB-EP430255STN
CSB-BP430255STN
CSB-MP430255STN
CSB-EP430255STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica N-succinylarginine dihydrolase (astB) CSB-YP430256STN
CSB-EP430256STN
CSB-BP430256STN
CSB-MP430256STN
CSB-EP430256STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Peptidase E (pepE) CSB-YP430257STN
CSB-EP430257STN
CSB-BP430257STN
CSB-MP430257STN
CSB-EP430257STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP430258STN
CSB-EP430258STN
CSB-BP430258STN
CSB-MP430258STN
CSB-EP430258STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Elongation factor 4 (lepA), partial CSB-YP430259STN
CSB-EP430259STN
CSB-BP430259STN
CSB-MP430259STN
CSB-EP430259STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phosphoheptose isomerase (gmhA) CSB-YP430260STN
CSB-EP430260STN
CSB-BP430260STN
CSB-MP430260STN
CSB-EP430260STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lemna minor ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP430261LLL1
CSB-EP430261LLL1
CSB-BP430261LLL1
CSB-MP430261LLL1
CSB-EP430261LLL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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