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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella paratyphi B Phosphomethylpyrimidine synthase (thiC), partial CSB-YP430462STF
CSB-EP430462STF
CSB-BP430462STF
CSB-MP430462STF
CSB-EP430462STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP430463STF
CSB-EP430463STF
CSB-BP430463STF
CSB-MP430463STF
CSB-EP430463STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Lipid-A-disaccharide synthase (lpxB) CSB-YP430464STF
CSB-EP430464STF
CSB-BP430464STF
CSB-MP430464STF
CSB-EP430464STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 3-dehydroquinate dehydratase (aroD) CSB-YP430465STF
CSB-EP430465STF
CSB-BP430465STF
CSB-MP430465STF
CSB-EP430465STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Maltoporin (lamB) CSB-YP430466STF
CSB-EP430466STF
CSB-BP430466STF
CSB-MP430466STF
CSB-EP430466STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP430467STF
CSB-EP430467STF
CSB-BP430467STF
CSB-MP430467STF
CSB-EP430467STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP430468STF
CSB-EP430468STF
CSB-BP430468STF
CSB-MP430468STF
CSB-EP430468STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Cytoskeleton protein rodZ (rodZ), partial CSB-YP430469STF
CSB-EP430469STF
CSB-BP430469STF
CSB-MP430469STF
CSB-EP430469STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0061 protein ydiU (ydiU) CSB-YP430470STF
CSB-EP430470STF
CSB-BP430470STF
CSB-MP430470STF
CSB-EP430470STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP430471STF
CSB-EP430471STF
CSB-BP430471STF
CSB-MP430471STF
CSB-EP430471STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B tRNA pseudouridine synthase D (truD) CSB-YP430472STF
CSB-EP430472STF
CSB-BP430472STF
CSB-MP430472STF
CSB-EP430472STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP430473STF
CSB-EP430473STF
CSB-BP430473STF
CSB-MP430473STF
CSB-EP430473STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Protein Xni (xni) CSB-YP430474STF
CSB-EP430474STF
CSB-BP430474STF
CSB-MP430474STF
CSB-EP430474STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Ethanolamine ammonia-lyase light chain (eutC) CSB-YP430475STF
CSB-EP430475STF
CSB-BP430475STF
CSB-MP430475STF
CSB-EP430475STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B tRNA-modifying protein ygfZ (ygfZ) CSB-YP430476STF
CSB-EP430476STF
CSB-BP430476STF
CSB-MP430476STF
CSB-EP430476STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP430477STF
CSB-EP430477STF
CSB-BP430477STF
CSB-MP430477STF
CSB-EP430477STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Biosynthetic arginine decarboxylase (speA), partial CSB-YP430478STF
CSB-EP430478STF
CSB-BP430478STF
CSB-MP430478STF
CSB-EP430478STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Uronate isomerase (uxaC) CSB-YP430479STF
CSB-EP430479STF
CSB-BP430479STF
CSB-MP430479STF
CSB-EP430479STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Iron-sulfur cluster repair protein YtfE (ytfE) CSB-YP430480STF
CSB-EP430480STF
CSB-BP430480STF
CSB-MP430480STF
CSB-EP430480STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Inosose dehydratase (iolE) CSB-YP430481STF
CSB-EP430481STF
CSB-BP430481STF
CSB-MP430481STF
CSB-EP430481STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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