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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bordetella petrii 50S ribosomal protein L6 (rplF) CSB-YP430982BPB
CSB-EP430982BPB
CSB-BP430982BPB
CSB-MP430982BPB
CSB-EP430982BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP430983BPB
CSB-EP430983BPB
CSB-BP430983BPB
CSB-MP430983BPB
CSB-EP430983BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 30S ribosomal protein S17 (rpsQ) CSB-YP430984BPB
CSB-EP430984BPB
CSB-BP430984BPB
CSB-MP430984BPB
CSB-EP430984BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L2 (rplB) CSB-YP430985BPB
CSB-EP430985BPB
CSB-BP430985BPB
CSB-MP430985BPB
CSB-EP430985BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L1 (rplA) CSB-YP430986BPB
CSB-EP430986BPB
CSB-BP430986BPB
CSB-MP430986BPB
CSB-EP430986BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Membrane protein insertase YidC (yidC), partial CSB-YP430987BPB1
CSB-EP430987BPB1
CSB-BP430987BPB1
CSB-MP430987BPB1
CSB-EP430987BPB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Ribose-5-phosphate isomerase A (rpiA) CSB-YP430988BPB
CSB-EP430988BPB
CSB-BP430988BPB
CSB-MP430988BPB
CSB-EP430988BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii GTPase Der (der) CSB-YP430989BPB
CSB-EP430989BPB
CSB-BP430989BPB
CSB-MP430989BPB
CSB-EP430989BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Acetylglutamate kinase (argB) CSB-YP430990BOS
CSB-EP430990BOS
CSB-BP430990BOS
CSB-MP430990BOS
CSB-EP430990BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP430991BOS
CSB-EP430991BOS
CSB-BP430991BOS
CSB-MP430991BOS
CSB-EP430991BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum GTPase obg (obg) CSB-YP430992BOS
CSB-EP430992BOS
CSB-BP430992BOS
CSB-MP430992BOS
CSB-EP430992BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Probable rRNA maturation factor (BT_0248) CSB-YP430993BOS
CSB-EP430993BOS
CSB-BP430993BOS
CSB-MP430993BOS
CSB-EP430993BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum DNA mismatch repair protein mutL (mutL), partial CSB-YP430994BOS
CSB-EP430994BOS
CSB-BP430994BOS
CSB-MP430994BOS
CSB-EP430994BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP430995BPB
CSB-EP430995BPB
CSB-BP430995BPB
CSB-MP430995BPB
CSB-EP430995BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L20 (rplT) CSB-YP430996BPB
CSB-EP430996BPB
CSB-BP430996BPB
CSB-MP430996BPB
CSB-EP430996BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Large-conductance mechanosensitive channel (mscL), partial CSB-YP430997BOS1
CSB-EP430997BOS1
CSB-BP430997BOS1
CSB-MP430997BOS1
CSB-EP430997BOS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Na (+)/H (+) antiporter nhaA, partial CSB-YP430998BOS
CSB-EP430998BOS
CSB-BP430998BOS
CSB-MP430998BOS
CSB-EP430998BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP430999BOS
CSB-EP430999BOS
CSB-BP430999BOS
CSB-MP430999BOS
CSB-EP430999BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Chaperone protein hscA homolog (hscA), partial CSB-YP431000BPB
CSB-EP431000BPB
CSB-BP431000BPB
CSB-MP431000BPB
CSB-EP431000BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP431001BPB
CSB-EP431001BPB
CSB-BP431001BPB
CSB-MP431001BPB
CSB-EP431001BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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