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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Delftia acidovorans DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP432663DHV
CSB-EP432663DHV
CSB-BP432663DHV
CSB-MP432663DHV
CSB-EP432663DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Probable rRNA maturation factor (Daci_1224) CSB-YP432664DHV
CSB-EP432664DHV
CSB-BP432664DHV
CSB-MP432664DHV
CSB-EP432664DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP432665DHV
CSB-EP432665DHV
CSB-BP432665DHV
CSB-MP432665DHV
CSB-EP432665DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Pantothenate synthetase (panC) CSB-YP432666DHV
CSB-EP432666DHV
CSB-BP432666DHV
CSB-MP432666DHV
CSB-EP432666DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Trans-aconitate 2-methyltransferase (tam) CSB-YP432667DHV
CSB-EP432667DHV
CSB-BP432667DHV
CSB-MP432667DHV
CSB-EP432667DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP432668DHV
CSB-EP432668DHV
CSB-BP432668DHV
CSB-MP432668DHV
CSB-EP432668DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Large-conductance mechanosensitive channel (mscL), partial CSB-YP432669DHV1
CSB-EP432669DHV1
CSB-BP432669DHV1
CSB-MP432669DHV1
CSB-EP432669DHV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Protein CyaY (cyaY) CSB-YP432670DHV
CSB-EP432670DHV
CSB-BP432670DHV
CSB-MP432670DHV
CSB-EP432670DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP432671DHV
CSB-EP432671DHV
CSB-BP432671DHV
CSB-MP432671DHV
CSB-EP432671DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Aspartate carbamoyltransferase (pyrB) CSB-YP432672DHV
CSB-EP432672DHV
CSB-BP432672DHV
CSB-MP432672DHV
CSB-EP432672DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Chromosome partition protein Smc (smc), partial CSB-YP432673DHV
CSB-EP432673DHV
CSB-BP432673DHV
CSB-MP432673DHV
CSB-EP432673DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 50S ribosomal protein L36 (rpmJ) CSB-YP432674DHV
CSB-EP432674DHV
CSB-BP432674DHV
CSB-MP432674DHV
CSB-EP432674DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Leucine--tRNA ligase (leuS), partial CSB-YP432675DHV
CSB-EP432675DHV
CSB-BP432675DHV
CSB-MP432675DHV
CSB-EP432675DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Ribosome-binding factor A (rbfA) CSB-YP432676DHV
CSB-EP432676DHV
CSB-BP432676DHV
CSB-MP432676DHV
CSB-EP432676DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans Threonine--tRNA ligase (thrS), partial CSB-YP432677DHV
CSB-EP432677DHV
CSB-BP432677DHV
CSB-MP432677DHV
CSB-EP432677DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens L-rhamnose mutarotase (rhaM) CSB-YP432678AYS
CSB-EP432678AYS
CSB-BP432678AYS
CSB-MP432678AYS
CSB-EP432678AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Urease accessory protein UreF (ureF) CSB-YP432679AYS
CSB-EP432679AYS
CSB-BP432679AYS
CSB-MP432679AYS
CSB-EP432679AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP432680AYS1
CSB-EP432680AYS1
CSB-BP432680AYS1
CSB-MP432680AYS1
CSB-EP432680AYS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Ribose 1,5-bisphosphate phosphokinase PhnN (phnN) CSB-YP432681AYS
CSB-EP432681AYS
CSB-BP432681AYS
CSB-MP432681AYS
CSB-EP432681AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative major head protein (orf44) CSB-YP432682SUN
CSB-EP432682SUN
CSB-BP432682SUN
CSB-MP432682SUN
CSB-EP432682SUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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