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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Coxiella burnetii Pantothenate kinase (coaA) CSB-YP431042DXO
CSB-EP431042DXO
CSB-BP431042DXO
CSB-MP431042DXO
CSB-EP431042DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Putative Holliday junction resolvase (CBUD_2194) CSB-YP431043DXO
CSB-EP431043DXO
CSB-BP431043DXO
CSB-MP431043DXO
CSB-EP431043DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S21 (rpsU) CSB-YP431044DXO
CSB-EP431044DXO
CSB-BP431044DXO
CSB-MP431044DXO
CSB-EP431044DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP431045DXO
CSB-EP431045DXO
CSB-BP431045DXO
CSB-MP431045DXO
CSB-EP431045DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Adenylate kinase (adk) CSB-YP431046DXO
CSB-EP431046DXO
CSB-BP431046DXO
CSB-MP431046DXO
CSB-EP431046DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP431047DXO
CSB-EP431047DXO
CSB-BP431047DXO
CSB-MP431047DXO
CSB-EP431047DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP431048DXO
CSB-EP431048DXO
CSB-BP431048DXO
CSB-MP431048DXO
CSB-EP431048DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UPF0234 protein CBUD_1993 (CBUD_1993) CSB-YP431049DXO
CSB-EP431049DXO
CSB-BP431049DXO
CSB-MP431049DXO
CSB-EP431049DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L25 (rplY) CSB-YP431050DXO
CSB-EP431050DXO
CSB-BP431050DXO
CSB-MP431050DXO
CSB-EP431050DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Methylglyoxal synthase (mgsA) CSB-YP431051DXO
CSB-EP431051DXO
CSB-BP431051DXO
CSB-MP431051DXO
CSB-EP431051DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UvrABC system protein C (uvrC), partial CSB-YP431052DXO
CSB-EP431052DXO
CSB-BP431052DXO
CSB-MP431052DXO
CSB-EP431052DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP431053DXO
CSB-EP431053DXO
CSB-BP431053DXO
CSB-MP431053DXO
CSB-EP431053DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L31 (rpmE) CSB-YP431054DXO
CSB-EP431054DXO
CSB-BP431054DXO
CSB-MP431054DXO
CSB-EP431054DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Protein RnfH (rnfH) CSB-YP431055DXO
CSB-EP431055DXO
CSB-BP431055DXO
CSB-MP431055DXO
CSB-EP431055DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii D-alanine--D-alanine ligase (ddl) CSB-YP431056DXO
CSB-EP431056DXO
CSB-BP431056DXO
CSB-MP431056DXO
CSB-EP431056DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phosphomethylpyrimidine synthase (thiC), partial CSB-YP431057DXO
CSB-EP431057DXO
CSB-BP431057DXO
CSB-MP431057DXO
CSB-EP431057DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP431058DXO
CSB-EP431058DXO
CSB-BP431058DXO
CSB-MP431058DXO
CSB-EP431058DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Glutamyl-tRNA reductase (hemA) CSB-YP431059DXO
CSB-EP431059DXO
CSB-BP431059DXO
CSB-MP431059DXO
CSB-EP431059DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Arginine--tRNA ligase (argS), partial CSB-YP431060DXO
CSB-EP431060DXO
CSB-BP431060DXO
CSB-MP431060DXO
CSB-EP431060DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Type III pantothenate kinase (coaX) CSB-YP431061CWZ
CSB-EP431061CWZ
CSB-BP431061CWZ
CSB-MP431061CWZ
CSB-EP431061CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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