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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pestis bv. Antiqua UPF0758 protein YpAngola_A0055 (YpAngola_A0055) CSB-YP431482YAM
CSB-EP431482YAM
CSB-BP431482YAM
CSB-MP431482YAM
CSB-EP431482YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Phosphopantetheine adenylyltransferase (coaD) CSB-YP431483YAM
CSB-EP431483YAM
CSB-BP431483YAM
CSB-MP431483YAM
CSB-EP431483YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Cell division protein ZapB (zapB) CSB-YP431484YAM
CSB-EP431484YAM
CSB-BP431484YAM
CSB-MP431484YAM
CSB-EP431484YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP431485YAM
CSB-EP431485YAM
CSB-BP431485YAM
CSB-MP431485YAM
CSB-EP431485YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP431486YAM
CSB-EP431486YAM
CSB-BP431486YAM
CSB-MP431486YAM
CSB-EP431486YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP431487YAM
CSB-EP431487YAM
CSB-BP431487YAM
CSB-MP431487YAM
CSB-EP431487YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 30S ribosomal protein S21 (rpsU) CSB-YP431488YAM
CSB-EP431488YAM
CSB-BP431488YAM
CSB-MP431488YAM
CSB-EP431488YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP431489YAM
CSB-EP431489YAM
CSB-BP431489YAM
CSB-MP431489YAM
CSB-EP431489YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP431490YAM
CSB-EP431490YAM
CSB-BP431490YAM
CSB-MP431490YAM
CSB-EP431490YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP431491YAM
CSB-EP431491YAM
CSB-BP431491YAM
CSB-MP431491YAM
CSB-EP431491YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP431492YAM
CSB-EP431492YAM
CSB-BP431492YAM
CSB-MP431492YAM
CSB-EP431492YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Regulator of sigma D (rsd) CSB-YP431493YAM
CSB-EP431493YAM
CSB-BP431493YAM
CSB-MP431493YAM
CSB-EP431493YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) CSB-YP431494YAM
CSB-EP431494YAM
CSB-BP431494YAM
CSB-MP431494YAM
CSB-EP431494YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0263 protein YpAngola_A2157 (YpAngola_A2157) CSB-YP431495YAM
CSB-EP431495YAM
CSB-BP431495YAM
CSB-MP431495YAM
CSB-EP431495YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP431496YAM
CSB-EP431496YAM
CSB-BP431496YAM
CSB-MP431496YAM
CSB-EP431496YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L2 (rplB) CSB-YP431497YAM
CSB-EP431497YAM
CSB-BP431497YAM
CSB-MP431497YAM
CSB-EP431497YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 30S ribosomal protein S4 (rpsD) CSB-YP431498YAM
CSB-EP431498YAM
CSB-BP431498YAM
CSB-MP431498YAM
CSB-EP431498YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0259 membrane protein YpAngola_A2324 (YpAngola_A2324) CSB-YP431499YAM
CSB-EP431499YAM
CSB-BP431499YAM
CSB-MP431499YAM
CSB-EP431499YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Cytochrome b mRNA maturase bI2 (bI2) CSB-YP431500DIS
CSB-EP431500DIS
CSB-BP431500DIS
CSB-MP431500DIS
CSB-EP431500DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATP synthase subunit 9, mitochondrial (ATP9), partial CSB-YP431501DIS1
CSB-EP431501DIS1
CSB-BP431501DIS1
CSB-MP431501DIS1
CSB-EP431501DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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