Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acholeplasma laidlawii Heat-inducible transcription repressor HrcA (hrcA) CSB-YP431422ATX
CSB-EP431422ATX
CSB-BP431422ATX
CSB-MP431422ATX
CSB-EP431422ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP431423ATX
CSB-EP431423ATX
CSB-BP431423ATX
CSB-MP431423ATX
CSB-EP431423ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii Isoleucine--tRNA ligase (ileS), partial CSB-YP431424ATX
CSB-EP431424ATX
CSB-BP431424ATX
CSB-MP431424ATX
CSB-EP431424ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii Alanine--tRNA ligase (alaS), partial CSB-YP431425ATX
CSB-EP431425ATX
CSB-BP431425ATX
CSB-MP431425ATX
CSB-EP431425ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii 30S ribosomal protein S2 (rpsB) CSB-YP431426ATX
CSB-EP431426ATX
CSB-BP431426ATX
CSB-MP431426ATX
CSB-EP431426ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii Probable tRNA sulfurtransferase (thiI) CSB-YP431427ATX
CSB-EP431427ATX
CSB-BP431427ATX
CSB-MP431427ATX
CSB-EP431427ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP431428ATX
CSB-BP431428ATX
CSB-MP431428ATX
CSB-EP431428ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea sitchensis CASP-like protein 7, partial CSB-YP431429EVO1
CSB-EP431429EVO1
CSB-BP431429EVO1
CSB-MP431429EVO1
CSB-EP431429EVO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea sitchensis Glutamyl-tRNA (Gln) amidotransferase subunit C, chloroplastic/mitochondrial, partial CSB-YP431430EVO
CSB-EP431430EVO
CSB-BP431430EVO
CSB-MP431430EVO
CSB-EP431430EVO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus Protein VP3, partial CSB-YP431431RIR
CSB-EP431431RIR
CSB-BP431431RIR
CSB-MP431431RIR
CSB-EP431431RIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachelium caeruleum ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP431432TOW1
CSB-EP431432TOW1
CSB-BP431432TOW1
CSB-MP431432TOW1
CSB-EP431432TOW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Probable GTP-binding protein EngB (engB) CSB-YP431433YAM
CSB-EP431433YAM
CSB-BP431433YAM
CSB-MP431433YAM
CSB-EP431433YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP431434YAM
CSB-EP431434YAM
CSB-BP431434YAM
CSB-MP431434YAM
CSB-EP431434YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Elongation factor P (efp) CSB-YP431435YAM
CSB-EP431435YAM
CSB-BP431435YAM
CSB-MP431435YAM
CSB-EP431435YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Rhamnulose-1-phosphate aldolase (rhaD) CSB-YP431436YAM
CSB-EP431436YAM
CSB-BP431436YAM
CSB-MP431436YAM
CSB-EP431436YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP431437YAM
CSB-EP431437YAM
CSB-BP431437YAM
CSB-MP431437YAM
CSB-EP431437YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Copper homeostasis protein CutC (cutC) CSB-YP431438YAM
CSB-EP431438YAM
CSB-BP431438YAM
CSB-MP431438YAM
CSB-EP431438YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Tyrosine--tRNA ligase (tyrS) CSB-YP431439YAM
CSB-EP431439YAM
CSB-BP431439YAM
CSB-MP431439YAM
CSB-EP431439YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Cysteine desulfuration protein sufE (sufE) CSB-YP431440YAM
CSB-EP431440YAM
CSB-BP431440YAM
CSB-MP431440YAM
CSB-EP431440YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Glutamate--tRNA ligase (gltX) CSB-YP431441YAM
CSB-EP431441YAM
CSB-BP431441YAM
CSB-MP431441YAM
CSB-EP431441YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>