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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus weihenstephanensis Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP431542BON
CSB-EP431542BON
CSB-BP431542BON
CSB-MP431542BON
CSB-EP431542BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ferredoxin--NADP reductase 1 (BcerKBAB4_0332) CSB-YP431543BON
CSB-EP431543BON
CSB-BP431543BON
CSB-MP431543BON
CSB-EP431543BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis DNA mismatch repair protein mutL (mutL), partial CSB-YP431544BON
CSB-EP431544BON
CSB-BP431544BON
CSB-MP431544BON
CSB-EP431544BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP431545BON
CSB-EP431545BON
CSB-BP431545BON
CSB-MP431545BON
CSB-EP431545BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis ATP synthase gamma chain (atpG) CSB-YP431546BON
CSB-EP431546BON
CSB-BP431546BON
CSB-MP431546BON
CSB-EP431546BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0059 membrane protein BcerKBAB4_5122 (BcerKBAB4_5122), partial CSB-YP431547BON1
CSB-EP431547BON1
CSB-BP431547BON1
CSB-MP431547BON1
CSB-EP431547BON1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0435 protein BcerKBAB4_0386 (BcerKBAB4_0386) CSB-YP431548BON
CSB-EP431548BON
CSB-BP431548BON
CSB-MP431548BON
CSB-EP431548BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S15 (rpsO) CSB-YP431549BON
CSB-EP431549BON
CSB-BP431549BON
CSB-MP431549BON
CSB-EP431549BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S2 (rpsB) CSB-YP431550BON
CSB-EP431550BON
CSB-BP431550BON
CSB-MP431550BON
CSB-EP431550BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0122 protein BcerKBAB4_3669 (BcerKBAB4_3669) CSB-YP431551BON
CSB-EP431551BON
CSB-BP431551BON
CSB-MP431551BON
CSB-EP431551BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit CSB-YP431552BON
CSB-EP431552BON
CSB-BP431552BON
CSB-MP431552BON
CSB-EP431552BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Antiholin-like protein LrgB (lrgB), partial CSB-YP431553BON
CSB-EP431553BON
CSB-BP431553BON
CSB-MP431553BON
CSB-EP431553BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S18 (rpsR) CSB-YP431554BON
CSB-EP431554BON
CSB-BP431554BON
CSB-MP431554BON
CSB-EP431554BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP431555BON
CSB-EP431555BON
CSB-BP431555BON
CSB-MP431555BON
CSB-EP431555BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP431556BON
CSB-EP431556BON
CSB-BP431556BON
CSB-MP431556BON
CSB-EP431556BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens DNA ligase (ligA), partial CSB-YP431557MNY
CSB-EP431557MNY
CSB-BP431557MNY
CSB-MP431557MNY
CSB-EP431557MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens D-alanine--D-alanine ligase (ddl) CSB-YP431558MNY
CSB-EP431558MNY
CSB-BP431558MNY
CSB-MP431558MNY
CSB-EP431558MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP431559MNY
CSB-EP431559MNY
CSB-BP431559MNY
CSB-MP431559MNY
CSB-EP431559MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 50S ribosomal protein L21 (rplU) CSB-YP431560MNY
CSB-EP431560MNY
CSB-BP431560MNY
CSB-MP431560MNY
CSB-EP431560MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Ketol-acid reductoisomerase (ilvC) CSB-YP431561MNY
CSB-EP431561MNY
CSB-BP431561MNY
CSB-MP431561MNY
CSB-EP431561MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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